| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93990-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93798-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92813-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37326-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52519-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61735-0.025ml | |||
![Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/EB3-Antibody-7J9V3-Western-Blot-NBP3-15904-img0004.jpg "Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112146..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112350..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112518..jpg)
![Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391] - Western blot analysis of extracts of various cell lines, using ARF6 Rabbit mAb (NBP3-15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ARF6-Antibody-9E7T9-Western-Blot-NBP3-15391-img0002.jpg "Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391] - Western blot analysis of extracts of various cell lines, using ARF6 Rabbit mAb (NBP3-15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: ARF6 Antibody (9E7T9) [NBP3-15391] - Immunofluorescence analysis of C6 cells using ARF6 Rabbit mAb (NBP3-15391) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ARF6-Antibody-9E7T9-Immunocytochemistry-Immunofluorescence-NBP3-15391-img0001.jpg)
![Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391] - Western blot analysis of extracts of various cell lines, using ARF6 Rabbit mAb (NBP3-15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ARF6-Antibody-9E7T9-Western-Blot-NBP3-15391-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ARF6 Antibody (9E7T9) [ARF6] - Immunofluorescence analysis of C6 cells using ARF6 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15391_rabbit-arf6-mab-9e7t9-52202517162346.jpg)
![Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.](http://images.novusbio.com/fullsize/Ninein-Antibody-Western-Blot-NBP2-93990-img0005.jpg "Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded rat kidney using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0004.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded human stomach using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0002.jpg)
![Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded mouse lung using Ninein .](http://images.novusbio.com/fullsize/Ninein-Antibody-Immunohistochemistry-Paraffin-NBP2-93990-img0003.jpg)
![Western Blot: DIS3 Antibody [NBP2-81804] - Analysis of DIS3 in human ovary tissue lysate with DIS3 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/DIS3-Antibody-Western-Blot-NBP2-81804-img0001.jpg "Western Blot: DIS3 Antibody [NBP2-81804] - Analysis of DIS3 in human ovary tissue lysate with DIS3 antibody at 1 ug/ml.")
![Immunohistochemistry: DIS3 Antibody - BSA Free [NBP2-81804] - Immunohistochemistry of DIS3 in mouse testis tissue with DIS3 antibody at 2.5 u/ml.](http://images.novusbio.com/fullsize/nbp2-81804_rabbit-polyclonal-dis3-antibody-2711202420352429.jpg)
![Immunocytochemistry/ Immunofluorescence: DIS3 Antibody - BSA Free [NBP2-81804] - Immunofluorescence of DIS3 in mouse testis tissue with DIS3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81804_rabbit-polyclonal-dis3-antibody-2711202420263219.jpg)
![Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Western-Blot-NBP2-93798-img0004.jpg "Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Rat kidney using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0003.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Human esophagus using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0001.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Mouse liver using /PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0002.jpg)
![Western Blot: 14-3-3 zeta/delta Antibody [NBP2-92813] - Analysis of extracts of various cell lines, using 14-3-3 zeta/delta at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/14-3-3-zeta-delta-Antibody-Western-Blot-NBP2-92813-img0003.jpg "Western Blot: 14-3-3 zeta/delta Antibody [NBP2-92813] - Analysis of extracts of various cell lines, using 14-3-3 zeta/delta at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta/delta Antibody [NBP2-92813] - Rat kidney using YWHAZ Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/14-3-3-zeta-delta-Antibody-Immunohistochemistry-Paraffin-NBP2-92813-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/delta Antibody - Azide and BSA Free [NBP2-92813] - Immunofluorescence analysis of paraffin-embedded Rat brain using 14-3-3 zeta/delta Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92813_rabbit-polyclonal-14-3-3-zeta-delta-antibody-42202514251835.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0004.jpg "Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.")
![Flow Cytometry: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Flow-Cytometry-NBP2-37326-img0005.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0002.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0003.jpg)
![ELISA: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-ELISA-NBP2-37326-img0001.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0005.jpg "Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis of paraffin-embedded ovarian cancer tissues using NME2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Immunohistochemistry-Paraffin-NBP2-52519-img0004.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - nalysis using NME2 mAb against HEK293 (1) and NME2 (AA: FULL(1-152))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0003.jpg)
![Western Blot: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Analysis using NME2 mAb against human NME2 (AA: FULL(1-152)) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-Western-Blot-NBP2-52519-img0002.jpg)
![ELISA: NM23-H2/NME2 Antibody (4G7A8) [NBP2-52519] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-4G7A8-ELISA-NBP2-52519-img0007.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), C6 (3), MCF-7 (4), SK-N-SH (5), NTERA-2 (6) cell lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0005.jpg "Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), C6 (3), MCF-7 (4), SK-N-SH (5), NTERA-2 (6) cell lysate.")
![Flow Cytometry: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Flow cytometric analysis of Hela cells using CSNK2B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Flow-Cytometry-NBP2-52450-img0001.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mAb against HEK293 (1) and CSNK2B (AA: FULL(1-215))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0003.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mAb against human CSNK2B (AA: FULL(1-215)) recombinant protein. (Expected MW is 35 kDa)](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0002.jpg)
![ELISA: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-ELISA-NBP2-52450-img0004.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0004.jpg "Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)")
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0005.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded colon cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0002.jpg)
![ELISA: CIRBP Antibody (6E12D5) [NBP2-61735] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-ELISA-NBP2-61735-img0001.jpg)
![Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Western-Blot-NBP2-70847-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0004.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0002.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0003.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0005.jpg)
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)