| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37496-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37495-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99017-50ul | |||
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0003.jpg "Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8) [NBP2-37496] - Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Flow-Cytometry-NBP2-37496-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0002.jpg)
![ELISA: RAP1A Antibody (5F8) [NBP2-37496] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-ELISA-NBP2-37496-img0001.jpg)
![Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against human recombinant ATP2C1.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Western-Blot-NBP1-47467-img0003.jpg "Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against human recombinant ATP2C1.")
![Immunohistochemistry: ATP2C1 Antibody (4G12) [NBP1-47467] - Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and breast cancer (right) tissues using ATP2C1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Immunohistochemistry-NBP1-47467-img0001.jpg)
![Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against A431 (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Western-Blot-NBP1-47467-img0002.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0003.jpg "Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8G2) [NBP2-37495] - Flow cytometric analysis of HeLa cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Flow-Cytometry-NBP2-37495-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 21 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0002.jpg)
![ELISA: RAP1A Antibody (5F8G2) [NBP2-37495] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-ELISA-NBP2-37495-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Western-Blot-NBP1-22410-img0005.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-3A10-C9-Immunocytochemistry-Immunofluorescence-NBP1-22410-img0006.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-27820241631646.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-29820241492678.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-2920241192361.jpg)
![Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-278202415295551.jpg "Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32199) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-298202413585510.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Flow cytometric analysis of Hela cells labeling HGFR/c-MET. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32199, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-29202416453259.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0004.jpg "Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in cynomolgus heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in human colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0002.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0003.jpg)
![Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa](http://images.novusbio.com/fullsize/PRC1-Antibody-Western-Blot-NBP2-99017-img0002.jpg "Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa")
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99017] - Immunochemical staining of human PRC1 in human testis with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99017-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99017] - Immunofluorescence staining of PRC1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99017 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99017_rabbit-polyclonal-prc1-antibody-235202511444419.png)
![Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-288202412493478.jpg "Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33022) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-298202414115428.jpg)
![Flow Cytometry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Flow cytometric analysis of HT-29 cells labeling TIMP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33022, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-392024884693.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-278202415242648.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-298202414333636.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-29202410334015.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in human liver.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0006.jpg "Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in human liver.")
![Immunocytochemistry/Immunofluorescence: SGTA Antibody [H00006449-B01P] - Analysis of purified antibody to SGTA on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/SGTA-Antibody-Immunocytochemistry-Immunofluorescence-H00006449-B01P-img0004.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in Jurkat.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0005.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in transfected 293T cell line by SGTA polyclonal antibody. Lane 1: SGTA transfected lysate(34.43 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0007.jpg)