| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59472-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03659-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92649-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37363-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52492-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22415-0.025mg | |||
![Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cdc23-Antibody-1Q2I3-Western-Blot-NBP3-16631-img0002.jpg "Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb (NBP3-16631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdc23-Antibody-1Q2I3-Immunocytochemistry-Immunofluorescence-NBP3-16631-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16631_rabbit-cdc23-mab-1q2i3-522025125457.jpg%3Ft=1688440202)
![Western Blot: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: PC3 cell lysates, Lane 4: HaCaT cell lysates, Lane 5: THP1 cell lysates, Lane 6: U87MG cell lysates, Lane 7: A427 cell lysates, Lane 8: SK-OV-3 cell lysates, Lane 9: A549 cell lysates](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Western-Blot-NBP2-59472-img0001.jpg "Western Blot: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: PC3 cell lysates, Lane 4: HaCaT cell lysates, Lane 5: THP1 cell lysates, Lane 6: U87MG cell lysates, Lane 7: A427 cell lysates, Lane 8: SK-OV-3 cell lysates, Lane 9: A549 cell lysates")
![Immunocytochemistry/Immunofluorescence: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-59472-img0002.jpg)
![Flow Cytometry: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Flow cytometry analysis of S100A11 in HeLa cells. The cell was stained with NBP2-59472 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Flow-Cytometry-NBP2-59472-img0005.jpg)
![ELISA: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Flow cytometry analysis of S100A11 in A549 cells. The cell was stained with NBP2-59472 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-ELISA-NBP2-59472-img0006.jpg)
![Western Blot: KRAS Antibody [NBP3-03659] - Analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/KRAS-Antibody-Knockout-Validated-NBP3-03659-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: KRAS Antibody [NBP3-03659] - Analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Polyclonal KRAS Antibody [NBP3-03659] - Analysis of various lysates, using KRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-03659_rabbit-polyclonal-kras-antibody-western-blot-542023103522.jpg)
![Immunocytochemistry/ Immunofluorescence: KRAS Antibody - Azide and BSA Free [NBP3-03659] - Immunofluorescence analysis of L929 cells using [KO Validated] KRAS Rabbit pAb (A12704) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03659_rabbit-polyclonal-kras-antibody-52202519302990.jpg)
![Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RCC1-Antibody-1F0C5-Western-Blot-NBP3-15295-img0002.jpg "Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295] - Human placenta using RCC1 Rabbit mAb (NBP3-15295) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RCC1-Antibody-1F0C5-Immunohistochemistry-Paraffin-NBP3-15295-img0001.jpg)
![Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/RCC1-Antibody-1F0C5-Western-Blot-NBP3-15295-img0003.jpg)
![Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Western-Blot-NBP2-92649-img0004.jpg "Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Rat testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Mouse testis using Cyclin G .mouse testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0002.jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis using FLT3 mAb against human FLT3 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Western-Blot-NBP2-37363-img0002.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis using FLT3 mAb against human FLT3 recombinant protein. (Expected MW is 32.6 kDa)")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis of liver cancer tissues using FLT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Immunohistochemistry-NBP2-37363-img0004.jpg)
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Analysis of cervical cancer tissues using FLT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-Immunohistochemistry-NBP2-37363-img0003.jpg)
![ELISA: Flt-3/Flk-2/CD135 Antibody (7B7C3) [NBP2-37363] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7B7C3-ELISA-NBP2-37363-img0001.jpg)
![Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Western-Blot-NBP2-52492-img0004.jpg "Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)")
![Flow Cytometry: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis of Hela cells using TARDBP mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Flow-Cytometry-NBP2-52492-img0001.jpg)
![Western Blot: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Analysis using TARDBP mAb against HEK293 (1) and TARDBP (AA: 126-260)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-Western-Blot-NBP2-52492-img0003.jpg)
![ELISA: TDP-43/TARDBP Antibody (7F9A6) [NBP2-52492] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-7F9A6-ELISA-NBP2-52492-img0005.jpg)
![Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against human recombinant ATP2C1.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Western-Blot-NBP1-47467-img0003.jpg "Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against human recombinant ATP2C1.")
![Immunohistochemistry: ATP2C1 Antibody (4G12) [NBP1-47467] - Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and breast cancer (right) tissues using ATP2C1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Immunohistochemistry-NBP1-47467-img0001.jpg)
![Western Blot: ATP2C1 Antibody (4G12) [NBP1-47467] - Western blot analysis using ATP2C1 mouse mAb against A431 (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/ATP2C1-Antibody-4G12-Western-Blot-NBP1-47467-img0002.jpg)
![Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Western-Blot-NBP2-22415-img0004.jpg "Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.")
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0003.jpg)
![Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-278202416285255.jpg "Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody (JE65-09) [NBP3-32192] - Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-2920241114835.jpg)
![Flow Cytometry: CLIC4 Antibody (JE65-09) [NBP3-32192] - Flow cytometric analysis of HeLa cells labeling CLIC4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32192, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-29202416372180.jpg)
![Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-2682024159394.jpg "Immunohistochemistry-Frozen: APP Antibody (JE30-54) [NBP3-32034] - Immunofluorescence analysis of frozen mouse cerebral cortex tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: APP Antibody (JE30-54) [NBP3-32034] - Western blot analysis of APP on different lysates with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-27820241524269.jpg)
![Immunohistochemistry: APP Antibody (JE30-54) [NBP3-32034] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP antibody (NBP3-32034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32034_rabbit-app-mab-je30-54-298202414304185.jpg)
![Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-278202415295551.jpg "Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32199) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-298202413585510.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Flow cytometric analysis of Hela cells labeling HGFR/c-MET. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32199, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-29202416453259.jpg)