| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38390-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38226-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61735-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37326-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61815-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12704-50ul | |||
![Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38390_rabbit-dbc1-p30-pab-912202415143612.jpg "Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: DBC1/p30 Antibody [NBP3-38390] - Western blot analysis of various lysates using DBC1/p30 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38390_rabbit-dbc1-p30-pab-912202415152894.jpg)
![Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38390_rabbit-dbc1-p30-pab-912202415152888.jpg)
![Immunohistochemistry: DBC1/p30 Antibody [NBP3-38390] - Immunohistochemistry analysis of paraffin-embedded Human rectal cancer using DBC1/p30 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38390_rabbit-dbc1-p30-pab-91220241581284.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415111899.jpg "Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: CIRBP Antibody [NBP3-38226] - Western blot analysis of lysates from Rat testis, using CIRBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121323.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human placenta using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121388.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Rat brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202414163069.jpg)
![Western Blot: PRC1 Antibody [NBP2-81882] - Analysis of PRC1 in human skeletal muscle tissue lysate with PRC1 antibody at (A) 0.5 ug/ml and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/PRC1-Antibody-Western-Blot-NBP2-81882-img0001.jpg "Western Blot: PRC1 Antibody [NBP2-81882] - Analysis of PRC1 in human skeletal muscle tissue lysate with PRC1 antibody at (A) 0.5 ug/ml and (B) 1 ug/ml.")
![Immunohistochemistry: PRC1 Antibody - BSA Free [NBP2-81882] - Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81882_rabbit-polyclonal-prc1-antibody-271120242022938.jpg)
![Immunocytochemistry/ Immunofluorescence: PRC1 Antibody - BSA Free [NBP2-81882] - Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81882_rabbit-polyclonal-prc1-antibody-2711202419241356.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0004.jpg "Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)")
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0005.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded colon cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0002.jpg)
![ELISA: CIRBP Antibody (6E12D5) [NBP2-61735] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-ELISA-NBP2-61735-img0001.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0004.jpg "Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.")
![Flow Cytometry: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Flow-Cytometry-NBP2-37326-img0005.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0002.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0003.jpg)
![ELISA: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-ELISA-NBP2-37326-img0001.jpg)
![Western Blot: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis using ADAMTS1 mAb against HEK293 (1) and ADAMTS1 (AA: 858-960)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-Western-Blot-NBP2-61815-img0004.jpg "Western Blot: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis using ADAMTS1 mAb against HEK293 (1) and ADAMTS1 (AA: 858-960)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis of K562 cells using ADAMTS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-Flow-Cytometry-NBP2-61815-img0007.jpg)
![Western Blot: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis using ADAMTS1 mAb against human ADAMTS1 (AA: 858-960) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-Western-Blot-NBP2-61815-img0003.jpg)
![Western Blot: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis using ADAMTS1 mouse mAb against Hela (1) cell lysate.](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-Western-Blot-NBP2-61815-img0005.jpg)
![Flow Cytometry: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Analysis of Hela cells using ADAMTS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-ELISA-NBP2-61815-img0002.jpg)
![ELISA: ADAMTS1 Antibody (1C12E6) [NBP2-61815] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ADAMTS1-Antibody-1C12E6-ELISA-NBP2-61815-img0001.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0004.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.")
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0001.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0002.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0003.jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Western-Blot-NBP2-73184-img0001.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73184-img0002.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [NBP3-12704] - Lane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa](http://images.novusbio.com/fullsize/alpha-N-terminal-Methyltransferase-1A-METTL11A-Antibody-Western-Blot-NBP3-12704-img0001.jpg "Western Blot: alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [NBP3-12704] - Lane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa")
![Immunohistochemistry-Paraffin: alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [NBP3-12704] - Staining of human METTL11A in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/alpha-N-terminal-Methyltransferase-1A-METTL11A-Antibody-Immunohistochemistry-Paraffin-NBP3-12704-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [NBP3-12704] - Staining of human METTL11A in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/alpha-N-terminal-Methyltransferase-1A-METTL11A-Antibody-Immunohistochemistry-Paraffin-NBP3-12704-img0002.jpg)
![Immunoprecipitation: alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [NBP3-12704] - Lane A:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-NTMT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NTMT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :25 kDa](http://images.novusbio.com/fullsize/alpha-N-terminal-Methyltransferase-1A-METTL11A-Antibody-Immunoprecipitation-NBP3-12704-img0004.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)