| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93616-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-86682 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47771-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26666-50ul | |||
![Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ATM-Antibody-6P6C6-Western-Blot-NBP3-15838-img0002.jpg "Western Blot: ATM Antibody (6P6C6) [NBP3-15838] - Western blot analysis of extracts of various cell lines, using ATM antibody (NBP3-15838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: ATM Antibody (6P6C6) [ATM] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATM Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15838_rabbit-atm-mab-6p6c6-52202517172899.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody (8C8A9) [NBP3-16395] - Western blot analysis of extracts of various cell lines, using 14-3-3 sigma/Stratifin Rabbit mAb (NBP3-16395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-8C8A9-Western-Blot-NBP3-16395-img0002.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody (8C8A9) [NBP3-16395] - Western blot analysis of extracts of various cell lines, using 14-3-3 sigma/Stratifin Rabbit mAb (NBP3-16395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (8C8A9) [NBP3-16395] - Immunohistochemistry of paraffin-embedded human colon using 14-3-3 sigma/Stratifin Rabbit mAb (NBP3-16395) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-8C8A9-Immunohistochemistry-Paraffin-NBP3-16395-img0001.jpg)
![Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04486-img0002.jpg "Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP3-04486] - Analysis of HeLa cells using HRAS antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04486-img0001.jpg)
![Western Blot: RBX1 Antibody [NBP2-93616] - Analysis of extracts of various cell lines, using RBX1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/RBX1-Antibody-Western-Blot-NBP2-93616-img0002.jpg "Western Blot: RBX1 Antibody [NBP2-93616] - Analysis of extracts of various cell lines, using RBX1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: RBX1 Antibody [NBP2-93616] - Analysis of MCF-7 cells using RBX1 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93616-img0001.jpg)
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) - IHC-Prediluted [NBP2-48089] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).](http://images.novusbio.com/fullsize/c-Myc-Antibody-SPM237-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48089-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) - IHC-Prediluted [NBP2-48089] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401] - Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-+-KIP1-769-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48401-img0001.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401] - Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)")
![Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Ets-1-Antibody-ETS1-1801-Azide-and-BSA-Free-Protein-Array-NBP2-54514-img0002.jpg "Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
![Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0058.jpg "Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0057.jpg)
![Western Blot: DBC1/p30 Antibody [NB200-189] - Detection of Human and Mouse DBC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DBC1 antibody NB200-189 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DBC1-p30-Antibody-Western-Blot-NB200-189-img0003.jpg "Western Blot: DBC1/p30 Antibody [NB200-189] - Detection of Human and Mouse DBC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DBC1 antibody NB200-189 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DBC1/p30 Antibody [NB200-189] - Detection of human DBC1/p30 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit DBC1/p30 Antibody NB200-189 (lot 2) used for IP at 6 ug per reaction. DBC1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-DBC1 antibody NB200-190. For blotting immunoprecipitated DBC1, NB200-189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/DBC1-p30-Antibody-Immunoprecipitation-NB200-189-img0004.jpg)
![Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-262202414471566.jpg "Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: CDK2 Antibody (6A5) [NBP3-26666] - Positive Western Blot detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate. All lanes: CDK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 31 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-282202411351622.jpg)