| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26278-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86881-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80960-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84946-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33450-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37968-20ul | |||
![Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-81864] - Analysis of Thyroid receptor-interacting protein 12 in rat colon tissue lysate with Thyroid receptor-interacting protein 12 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Western-Blot-NBP2-81864-img0001.jpg "Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-81864] - Analysis of Thyroid receptor-interacting protein 12 in rat colon tissue lysate with Thyroid receptor-interacting protein 12 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunofluorescence of Thyroid receptor-interacting protein 12 in rat colon tissue with Thyroid receptor-interacting protein 12 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202419562160.jpg)
![Immunocytochemistry/ Immunofluorescence: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunocytochemistry of Thyroid receptor-interacting protein 12 in Hela cells with Thyroid receptor-interacting protein 12 Antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202420324860.gif)
![Immunohistochemistry: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunohistochemistry of Thyroid receptor-interacting protein 12 in rat colon tissue with Thyroid receptor-interacting protein 12 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202420285888.jpg)
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2622024132372.jpg "Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-26220241447153.jpg)
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2722024856319.jpg)
![Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate. All lanes: Topoisomerase I Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 91 kDa Observed band size: 91 kDa](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-28220241144269.jpg)
![Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Overlay histogram showing HepG2 cells stained with Topoisomerase I Antibody (6D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-282202414312734.jpg)
![Western Blot: CDC37 Antibody (3C7) [H00011140-M01] - CDC37 monoclonal antibody (M01), clone 3C7. Analysis of CDC37 expression in HL-60.](http://images.novusbio.com/fullsize/CDC37-Antibody-3C7-Western-Blot-H00011140-M01-img0009.jpg "Western Blot: CDC37 Antibody (3C7) [H00011140-M01] - CDC37 monoclonal antibody (M01), clone 3C7. Analysis of CDC37 expression in HL-60.")
![Immunocytochemistry/Immunofluorescence: CDC37 Antibody (3C7) [H00011140-M01] - Analysis of monoclonal antibody to CDC37 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDC37-Antibody-3C7-Immunocytochemistry-Immunofluorescence-H00011140-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody (3C7) [H00011140-M01] - Analysis of monoclonal antibody to CDC37 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CDC37-Antibody-3C7-Immunohistochemistry-Paraffin-H00011140-M01-img0008.jpg)
![Western Blot: CDC37 Antibody (3C7) [H00011140-M01] - Analysis of CDC37 expression in transfected 293T cell line by CDC37 monoclonal antibody (M01), clone 3C7.Lane 1: CDC37 transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDC37-Antibody-3C7-Western-Blot-H00011140-M01-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: USP16 Antibody [NBP1-86881] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86881-img0007.jpg "Immunocytochemistry/Immunofluorescence: USP16 Antibody [NBP1-86881] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0011.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0008.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0009.jpg)
![Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/USP16-Antibody-Immunohistochemistry-Paraffin-NBP1-86881-img0010.jpg)
![Western Blot: CDC37 Antibody [NBP1-80960] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP1-80960-img0008.jpg "Western Blot: CDC37 Antibody [NBP1-80960] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: CDC37 Antibody [NBP1-80960] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80960-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP1-80960] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP1-80960-img0001.jpg)
![Western Blot: UbcH3/Cdc34 Antibody [NBP1-84946] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-112](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Western-Blot-NBP1-84946-img0005.jpg "Western Blot: UbcH3/Cdc34 Antibody [NBP1-84946] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-112")
![Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-84946] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84946-img0006.jpg)
![Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody [NBP1-84946] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunohistochemistry-Paraffin-NBP1-84946-img0001.jpg)
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining in human testis and pancreas tissues using anti-TRIP12 antibody. Corresponding TRIP12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-33450-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining in human testis and pancreas tissues using anti-TRIP12 antibody. Corresponding TRIP12 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-NBP2-33450-img0001.jpg)
![Immunohistochemistry: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining of urinary bladder](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-NBP2-33450-img0002.jpg)
![Immunohistochemistry: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining of testis cancer.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-NBP2-33450-img0003.jpg)
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-33450-img0004.jpg)
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-33450] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-33450-img0005.jpg)
![Western Blot: Rpn2 Antibody [NB120-2941] - Analysis of proteasome 19S subunit S1 from HeLa cell extract.](http://images.novusbio.com/fullsize/Rpn2-Antibody-Western-Blot-NB120-2941-img0003.jpg "Western Blot: Rpn2 Antibody [NB120-2941] - Analysis of proteasome 19S subunit S1 from HeLa cell extract.")
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411545578.jpg "Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: Cullin 1 Antibody [NBP3-37968] - Western blot analysis of various lysates, using Cullin 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241243916.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411572689.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241252829.jpg)
![Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-7W3E3-Western-Blot-NBP3-15821-img0004.jpg "Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded rat brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512435347.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded mouse brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512432070.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded human colon carcinoma tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512443371.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)