| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47811-0.1mg | |||
| IHC-Prediluted | NBP2-48037 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57891-25ul | |||
![Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-99081-img0002.jpg "Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-99081-img0001.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98433 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98433_rabbit-polyclonal-e-cadherin-antibody-23520252135138.png)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Western-Blot-H00051343-M09-img0003.jpg "Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).")
![Sandwich ELISA: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Detection limit for recombinant GST tagged FZR1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Sandwich-ELISA-H00051343-M09-img0002.jpg)
![Western Blot: APRIN Antibody [NBP2-15436] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/APRIN-Antibody-Western-Blot-NBP2-15436-img0002.jpg "Western Blot: APRIN Antibody [NBP2-15436] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: APRIN Antibody [NBP2-15436] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/APRIN-Antibody-Western-Blot-NBP2-15436-img0001.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Tonsil stained with CDC20 Monoclonal Antibody (CDC20/1102)](http://images.novusbio.com/fullsize/Cdc20-Antibody-CDC20-1102-Immunohistochemistry-Paraffin-NBP2-44407-img0002.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Tonsil stained with CDC20 Monoclonal Antibody (CDC20/1102)")
![Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (CDC20/1102)](http://images.novusbio.com/fullsize/Cdc20-Antibody-CDC20-1102-Immunohistochemistry-Paraffin-NBP2-44407-img0001.jpg)
![Western Blot: Septin-3 Antibody [NBP2-20302] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Western-Blot-NBP2-20302-img0002.jpg "Western Blot: Septin-3 Antibody [NBP2-20302] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Septin-3 Antibody [NBP2-20302] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Western-Blot-NBP2-20302-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: POT1 Antibody [NBP2-57891] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/POT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57891-img0001.jpg "Immunocytochemistry/Immunofluorescence: POT1 Antibody [NBP2-57891] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
