| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73857 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37822-0.025mg | |||
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249403838.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249393545.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249432868.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362777.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-212024939176.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249371566.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362730.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249355541.jpg "Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-212024941458.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249383130.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249355586.jpg)
![Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - GSPT1 antibody [HL1346] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249351367.jpg)
![Western Blot: GSPT1 Antibody (HL1346) - Azide and BSA Free [NBP3-25493] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-2120249342767.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1346] (NBP3-25493) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25493_rabbit-gspt1-mab-hl1346-azide-and-bsa-free-western-blot-141120251842300.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0007.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0002.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0006.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249374982.jpg "Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249383186.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249355536.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249403876.jpg)
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024937157.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249415929.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024941337.jpg)
![Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Western-Blot-NBP2-45570-img0013.jpg "Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.")
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Flow-Cytometry-NBP2-45570-img0001.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0002.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0003.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0004.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0005.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0006.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0007.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0008.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0009.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0010.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0011.jpg)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0022.jpg "Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.")
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0021.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0023.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0001.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0002.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0003.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0004.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0005.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0006.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0007.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0008.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0009.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0012.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0013.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0014.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0015.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0016.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0017.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0018.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0019.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0020.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Western-Blot-NBP1-47757-img0019.jpg "Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0020.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0011.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0012.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0013.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0014.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0015.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0017.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0018.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0011.jpg "Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0013.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0005.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0012.jpg)