| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38362-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37989-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61898-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06477-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99631-50ul | |||
![Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125530.png "Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.")
![Analysis of various lysates using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125718.png)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [p16INK4a/CDKN2A] - Confocal imaging of HeLa cells using [KO Validated] p16INK4a/CDKN2A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-52202517284956.jpg)
![Western Blot: COP9 Antibody [NBP3-38362] - Western blot analysis of various lysates using COP9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38362_rabbit-cop9-pab-101220241136022.jpg "Western Blot: COP9 Antibody [NBP3-38362] - Western blot analysis of various lysates using COP9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: COP9 Antibody [NBP3-38362] - Immunofluorescence analysis of MCF7 cells using COP9 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38362_rabbit-cop9-pab-1012202411353090.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-10122024121033.jpg "Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: CTBP2 Antibody [NBP3-37989] - Western blot analysis of various lysates using CTBP2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-101220241233858.jpg)
![Western Blot: TdT Antibody (4B10C3) [NBP2-61898] - Analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TdT-Antibody-4B10C3-Western-Blot-NBP2-61898-img0003.jpg "Western Blot: TdT Antibody (4B10C3) [NBP2-61898] - Analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: TdT Antibody (4B10C3) [NBP2-61898] - Analysis using DNTT mAb against human DNTT (AA: 52-192) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/TdT-Antibody-4B10C3-Western-Blot-NBP2-61898-img0002.jpg)
![Western Blot: TdT Antibody (4B10C3) [NBP2-61898] - Analysis using DNTT mouse mAb against MOLT4 (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/TdT-Antibody-4B10C3-Western-Blot-NBP2-61898-img0004.jpg)
![ELISA: TdT Antibody (4B10C3) [NBP2-61898] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TdT-Antibody-4B10C3-ELISA-NBP2-61898-img0001.jpg)
![Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Western-Blot-NBP3-06477-img0001.jpg "Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa")
![Immunoprecipitation: MNAT1 Antibody [NBP3-06477] - Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-MNAT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MNAT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :35 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Immunoprecipitation-NBP3-06477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MNAT1 Antibody [NBP3-06477] - Staining of MNAT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06477 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06477_rabbit-polyclonal-mnat1-antibody-235202511444422.png)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP2-82268] - WB Suggested Anti-SUV39H1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP2-82268-img0003.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP2-82268] - WB Suggested Anti-SUV39H1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in HepG2")
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP2-82268] - Rabbit Anti-SUV39H1 Antibody. Paraffin Embedded Tissue: Human alveolar cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP2-82268-img0002.jpg)
![Immunohistochemistry: KMT1A/SUV39H1 Antibody [NBP2-82268] - Human Intestine](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-NBP2-82268-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-278202416331268.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32689) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-298202414333625.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunocytochemistry analysis of SKOV-3 cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-29202410484819.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-99631-img0005.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-99631-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99631 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99631_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520251433152.png)
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)
![Western Blot: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Jurkat (A) (0.03µg/ml) and Molt-4 (B) (0.03µg/ml) cells lysates, and Jurkat (C) (0.01µg/ml) cells nuclear lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with NBP3-43576 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-western-blot-2220260322010.png "Western Blot: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Jurkat (A) (0.03µg/ml) and Molt-4 (B) (0.03µg/ml) cells lysates, and Jurkat (C) (0.01µg/ml) cells nuclear lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with NBP3-43576 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.")
![Flow Cytometry: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or tNBP3-43576 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-flow-cytometry-222026033316.png)
![Immunocytochemistry/ Immunofluorescence: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on plus coverslips, permeabilized with 0.15% Triton, stained with NBP3-43576 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43576, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2220260322023.png)
![Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-18920231735526.jpg "Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.")
![Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] - Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-CLASP2 (NBP3-23220) (middle). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-199202312523627.jpg)