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![Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791] - Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (NBP3-16791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MCM4-Antibody-5N0X3-Western-Blot-NBP3-16791-img0001.jpg "Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791] - Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (NBP3-16791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: RRM2 Antibody (10Y6E4) [NBP3-16182] - Western blot analysis of extracts of various cell lines, using RRM2 Rabbit mAb (NBP3-16182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RRM2-Antibody-10Y6E4-Western-Blot-NBP3-16182-img0001.jpg "Western Blot: RRM2 Antibody (10Y6E4) [NBP3-16182] - Western blot analysis of extracts of various cell lines, using RRM2 Rabbit mAb (NBP3-16182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: E2F5 Antibody [NBP2-82955] - WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/E2F5-Antibody-Western-Blot-NBP2-82955-img0001.jpg "Western Blot: E2F5 Antibody [NBP2-82955] - WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate")
![Western Blot: E2F5 Antibody [NBP2-82955] - Host: Rabbit. Target Name: E2F5. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/E2F5-Antibody-Western-Blot-NBP2-82955-img0002.jpg)
![Immunohistochemistry: HIC5/TGFB1I1 Antibody [NBP3-10498] - Immunohistochemical analysis of human tonsil lysate tissue at an antibody concentration of 5.0ug/ml](http://images.novusbio.com/fullsize/HIC5-TGFB1I1-Antibody-Immunohistochemistry-NBP3-10498-img0001.jpg "Immunohistochemistry: HIC5/TGFB1I1 Antibody [NBP3-10498] - Immunohistochemical analysis of human tonsil lysate tissue at an antibody concentration of 5.0ug/ml")
![Immunohistochemistry: HIC5/TGFB1I1 Antibody [NBP3-10300] - Immunohistochemical analysis of human lung.](http://images.novusbio.com/fullsize/HIC5-TGFB1I1-Antibody-Immunohistochemistry-NBP3-10300-img0001.jpg "Immunohistochemistry: HIC5/TGFB1I1 Antibody [NBP3-10300] - Immunohistochemical analysis of human lung.")
![Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_rabbit-mdm2-hdm2-p-ser166-mab-je64-20-288202412461655.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DDX24 Antibody (A3B10-R) [NBP3-32262] - Immunofluorescence analysis of paraffin-embedded zebrafish (head) tissue labeling DDX24 with Mouse anti-DDX24 antibody (NBP3-32262) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32262, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32262_mouse-ddx24-mab-a3b10-r-298202414161833.jpg "Immunohistochemistry: DDX24 Antibody (A3B10-R) [NBP3-32262] - Immunofluorescence analysis of paraffin-embedded zebrafish (head) tissue labeling DDX24 with Mouse anti-DDX24 antibody (NBP3-32262) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32262, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: DDX24 Antibody (A3B10-R) [NBP3-32262] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue with Mouse anti-DDX24 antibody (NBP3-32262) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32262_mouse-ddx24-mab-a3b10-r-298202414262642.jpg)
![Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NBP2-98759-img0002.jpg "Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa")
![Immunoprecipitation: HDAC7 Antibody [NBP2-98759] - HDAC7 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-HDAC7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HDAC7 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NBP2-98759-img0001.jpg)
![Western Blot: IFRD1 Antibody [NBP2-85090] - WB Suggested Anti-IFRD1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-85090-img0001.jpg "Western Blot: IFRD1 Antibody [NBP2-85090] - WB Suggested Anti-IFRD1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart")
![Western Blot: IFRD1 Antibody [NBP2-85090] - Host: Rabbit. Target Name: IFRD1. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-85090-img0002.jpg)
![Immunoprecipitation: NEK1 Antibody [NBP2-36533] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody NBP2-36533 used for IP at 6 ug per reaction. NEK1 was also immunoprecipitated by rabbit anti-NEK1 antibody NBP2-36534. For blotting immunoprecipitated NEK1, NBP2-36534 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunoprecipitation-NBP2-36533-img0001.jpg "Immunoprecipitation: NEK1 Antibody [NBP2-36533] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody NBP2-36533 used for IP at 6 ug per reaction. NEK1 was also immunoprecipitated by rabbit anti-NEK1 antibody NBP2-36534. For blotting immunoprecipitated NEK1, NBP2-36534 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")