| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58081-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49384-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35448-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16925-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94154-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: KLF11 Antibody [NBP2-58081] - Staining of human cell line SiHa shows localization to nucleoplasm & focal adhesion sites.](http://images.novusbio.com/fullsize/KLF11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58081-img0001.jpg "Immunocytochemistry/Immunofluorescence: KLF11 Antibody [NBP2-58081] - Staining of human cell line SiHa shows localization to nucleoplasm & focal adhesion sites.")
![Immunohistochemistry-Paraffin: IRF1 Antibody [NBP2-49384] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/IRF1-Antibody-Immunohistochemistry-NBP2-49384-img0001.jpg "Immunohistochemistry-Paraffin: IRF1 Antibody [NBP2-49384] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.")
![Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).](http://images.novusbio.com/fullsize/nbp3-30779_rabbit-ppp2r5c-pab-811202420592180.jpg "Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).")
![Western Blot: LZTS2 Antibody [NBP3-35448] - Western blot analysis of various lysates using LZTS2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35448_rabbit-lzts2-pab-912202414201362.jpg "Western Blot: LZTS2 Antibody [NBP3-35448] - Western blot analysis of various lysates using LZTS2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/TSC2-[p-Ser1387]-Antibody-Western-Blot-NBP3-16925-img0001.jpg "Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Western-Blot-NBP3-15763-img0001.jpg "Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSC2 antibody (NBP3-15763). Western blot was performed from the immunoprecipitate using TSC2 antibody (NBP3-15763) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Immunoprecipitation-NBP3-15763-img0002.jpg)
![Western Blot: PPP2R5C Antibody [NBP2-94154] - Analysis of extracts of various cell lines, using PPP2R5C at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP2-94154-img0004.jpg "Western Blot: PPP2R5C Antibody [NBP2-94154] - Analysis of extracts of various cell lines, using PPP2R5C at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Immunoprecipitation-NB100-1544-img0001.jpg "Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: IRF1 Antibody [NBP1-78761] - Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes.](http://images.novusbio.com/fullsize/IRF1-Antibody-Western-Blot-NBP1-78761-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IRF1 Antibody [NBP1-78761] - Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes.")