| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99003-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47811-0.1mg | |||
| IHC-Prediluted | NBP2-48037 | |||
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0003.jpg "Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0002.jpg)
![Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-99081-img0002.jpg "Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-99081-img0001.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98433 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98433_rabbit-polyclonal-e-cadherin-antibody-23520252135138.png)
![Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Western-Blot-H00051343-M09-img0003.jpg "Western Blot: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Analysis of FZR1 expression in HeLa (Cat # L013V1).")
![Sandwich ELISA: FZR1/CDH1 Antibody (3E12) [H00051343-M09] - Detection limit for recombinant GST tagged FZR1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-3E12-Sandwich-ELISA-H00051343-M09-img0002.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Tonsil stained with CDC20 Monoclonal Antibody (CDC20/1102)](http://images.novusbio.com/fullsize/Cdc20-Antibody-CDC20-1102-Immunohistochemistry-Paraffin-NBP2-44407-img0002.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Tonsil stained with CDC20 Monoclonal Antibody (CDC20/1102)")
![Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) [NBP2-44407] - Human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (CDC20/1102)](http://images.novusbio.com/fullsize/Cdc20-Antibody-CDC20-1102-Immunohistochemistry-Paraffin-NBP2-44407-img0001.jpg)
![Western Blot: Cdc27 Antibody [NBP2-15829] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC27 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Cdc27-Antibody-Western-Blot-NBP2-15829-img0002.jpg "Western Blot: Cdc27 Antibody [NBP2-15829] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC27 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunoprecipitation: CLASP2 Antibody [NBP1-21394] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CLASP2 antibody used for IP at 3 mcg/mg lysate. CLASP2 was also immunoprecipitated by rabbit anti-CLASP2 antibody NBP1-21395, which recognizes a downstream epitope](http://images.novusbio.com/fullsize/CLASP2-Antibody-Immunoprecipitation-NBP1-21394-img0002.jpg "Immunoprecipitation: CLASP2 Antibody [NBP1-21394] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CLASP2 antibody used for IP at 3 mcg/mg lysate. CLASP2 was also immunoprecipitated by rabbit anti-CLASP2 antibody NBP1-21395, which recognizes a downstream epitope")