| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92924-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03740-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52447-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB1580-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
![Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NBP2-92924-img0009.jpg "Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Rat testis using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0008.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Human tonsil using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0006.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Mouse spleen using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody [NBP2-92924] - Analysis of NIH-3T3 cells using CAD at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92924-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody - BSA Free [NBP2-92924] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92924_rabbit-polyclonal-cad-antibody-4220251429027.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251638096.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: NF2/Merlin Antibody [NBP3-03740] - Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2/Merlin antibody (NBP3-03740). Western blot was performed from the immunoprecipitate using NF2/Merlin antibody (NBP3-03740) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunoprecipitation-NBP3-03740-img0003.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of various lysates using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251634529.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of C6 cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03740-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of U2OS cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-52202516283287.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of L929 cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251628043.jpg)
![Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Western-Blot-NBP2-52447-img0004.jpg "Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (7E4F7) [NBP2-52447] - Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunofluorescence-NBP2-52447-img0001.jpg)
![Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of paraffin-embedded esophageal cancer tissues using CSF3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunohistochemistry-NBP2-52447-img0003.jpg)
![Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Western-Blot-NBP2-52447-img0005.jpg)
![Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-Immunohistochemistry-NBP2-52447-img0002.jpg)
![ELISA: G-CSF Antibody (7E4F7) [NBP2-52447] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-CSF-Antibody-7E4F7-ELISA-NBP2-52447-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310] - Cultured Rat hippocampal neurons, courtesy of customer.](http://images.novusbio.com/fullsize/Ezrin-Antibody-3C12-Immunocytochemistry-Immunofluorescence-NB600-1310-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310] - Cultured Rat hippocampal neurons, courtesy of customer.")
![Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded lung adenocarcinoma stained with Ezrin antibody (NB600-1310).](http://images.novusbio.com/fullsize/Ezrin-Antibody-3C12-Immunohistochemistry-Paraffin-NB600-1310-img0004.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded human lung stained with Ezrin antibody.](http://images.novusbio.com/fullsize/Ezrin-Antibody-3C12-Immunohistochemistry-Paraffin-NB600-1310-img0001.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0002.jpg "Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/JWA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12158-img0003.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Small Intestine with antibody at 3.75 g/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0006.jpg)
![Flow Cytometry: JWA Antibody [NBP3-12158] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/JWA-Antibody-Flow-Cytometry-NBP3-12158-img0004.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0001.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Tonsil with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunocytochemistry-Immunofluorescence-NBP1-97688-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688] - Analysis of human colon.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunohistochemistry-Paraffin-NBP1-97688-img0004.jpg)
![Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688] - Frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunohistochemistry-Frozen-NBP1-97688-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Immunohistochemistry-Paraffin-MAB1580-img0001.jpg "Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.")
![Simple Western: HSP27 Antibody (967050) [MAB1580] - MCF-7 cell lysate. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Simple-Western-MAB1580-img0002.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BLBP antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-16399-img0005.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BLBP antibody [N1C3] diluted at 1:1000.")
![Immunohistochemistry-Frozen: FABP7/B-FABP Antibody [NBP2-16399] - Frozen sectioned E13.5 Rat brain. Green: BLBP protein stained by BLBP antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Frozen-NBP2-16399-img0004.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-16399-img0002.jpg)
![FABP7 antibody [N1C3] detects FABP7 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FABP7 stained by FABP7 antibody [N1C3] (NBP2-16399) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16399_rabbit-polyclonal-fabp7-b-fabp-antibody-immunocytochemistry-immunofluorescence-2611202518283520.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Western-Blot-NB110-40548-img0005.jpg "Western Blot: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Pescadillo Antibody [NB110-40548] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pescadillo used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunohistochemistry-NB110-40548-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Pescadillo Antibody [NB110-40548] - Detection of Human Pescadillo on HeLa whole cell lysate using NB110-40548. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody and NB110-40548.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Western-Blot-NB110-40548-img0002.jpg)
![Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-Pescadillo used at a dilution of 1:250.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunohistochemistry-Paraffin-NB110-40548-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for IP at 3 ug per reaction. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody. For blotting immunoprecipitated Pescadillo, NB110-40548 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunoprecipitation-NB110-40548-img0006.jpg)
![Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Western-Blot-NBP2-15670-img0001.jpg "Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15670] - Confocal immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15670-img0003.jpg)
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15670-img0002.jpg)
![Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15670 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15670_rabbit-polyclonal-calpain-1-antibody-91020242091427.jpg)
![Immunohistochemistry: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (NBP2-15670) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-15670_rabbit-polyclonal-calpain-1-antibody-9102024202466.jpg)