| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05409-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92649-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35328-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04117-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38168-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38396-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06560-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98336-50ul | |||
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-05409-img0001.jpg "Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).")
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] - analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-western-blot-159202383355..jpg)
![Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] - Western blot analysis of lysates from C6 cells using CDK2 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-52202516345255.jpg)
![Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Western-Blot-NBP2-92649-img0004.jpg "Western Blot: Cyclin G Antibody [NBP2-92649] - Analysis of extracts of various cell lines, using Cyclin G1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Rat testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin G Antibody [NBP2-92649] - Mouse testis using Cyclin G .mouse testis using Cyclin G1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Cyclin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-92649-img0002.jpg)
![Western Blot: Cyclin D2 Antibody [NBP3-35328] - Western blot analysis of lysates from RD cells, using Cyclin D2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35328_rabbit-cyclin-d2-pab-912202415121363.jpg "Western Blot: Cyclin D2 Antibody [NBP3-35328] - Western blot analysis of lysates from RD cells, using Cyclin D2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: Cyclin D2 Antibody [NBP3-35328] - Immunofluorescence analysis of U2OS cells using Cyclin D2 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35328_rabbit-cyclin-d2-pab-912202414213780.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0002.jpg "Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Skp2 Antibody (2L0K1) [NBP3-16320] - Immunohistochemistry of paraffin-embedded human colon using Skp2 Rabbit mAb (NBP3-16320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Immunohistochemistry-Paraffin-NBP3-16320-img0001.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of various cell lines, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0003.jpg)
![Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04117-img0003.jpg "Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric cancer using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0002.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-91220241581241.jpg "Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: MCM5 Antibody [NBP3-38168] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202415111841.jpg)
![Western Blot: MCM5 Antibody [NBP3-38168] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202414112858.jpg)
![Western Blot: CDC16 Antibody [NBP3-38396] - Western blot analysis of various lysates using CDC16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-91220241517115.jpg "Western Blot: CDC16 Antibody [NBP3-38396] - Western blot analysis of various lysates using CDC16 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: CDC16 Antibody [NBP3-38396] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CDC16 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-9122024158123.jpg)
![Immunohistochemistry: CDC16 Antibody [NBP3-38396] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using CDC16 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38396_rabbit-cdc16-pab-912202414101810.jpg)
![Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cdc23-Antibody-1Q2I3-Western-Blot-NBP3-16631-img0002.jpg "Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb (NBP3-16631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdc23-Antibody-1Q2I3-Immunocytochemistry-Immunofluorescence-NBP3-16631-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16631_rabbit-cdc23-mab-1q2i3-522025125457.jpg%3Ft=1688440202)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0003.jpg "Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with testis at 5.2 ug/ml.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-NLS7979-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0002.jpg)
![Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Western-Blot-NBP3-06560-img0001.jpg "Western Blot: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa")
![Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP3-06560] - Staining of human CDC34 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunohistochemistry-Paraffin-NBP3-06560-img0002.jpg)
![Immunoprecipitation: UBE2R1/CDC34 Antibody [NBP3-06560] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-CDC34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa](http://images.novusbio.com/fullsize/UBE2R1-CDC34-Antibody-Immunoprecipitation-NBP3-06560-img0003.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98336-img0004.jpg "Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-98336] - Immunochemical staining of mouse E-Cadherin in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-98336-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP2-98336] - Mouse E-Cadherin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Mouse E-Cadherin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse E-Cadherin rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP2-98336-img0001.jpg)