| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06419-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97772-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06557-50ul | |||
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0003.jpg "Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa")
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 antibody at 1:500 dilution. Lane A: HL60 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lane D: Daudi Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC5 Antibody [NBP3-06419] - Immunofluorescence staining of CCDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06419 antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06419_rabbit-polyclonal-ccdc5-antibody-23520252135125.png)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)
![Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-27820241631632.jpg "Western Blot: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Western blot analysis of p18INK4c/CDKN2C on different lysates with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 6: Rat skeletal muscle tissue lysate (40 ug/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p18INK4c/CDKN2C Antibody (JE36-09) [NBP3-32690] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-p18INK4c/CDKN2C antibody (NBP3-32690) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32690) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32690_rabbit-p18ink4c-cdkn2c-mab-je36-09-298202414115420.jpg)
![Immunocytochemistry/ Immunofluorescence: SON Antibody - BSA Free [NBP3-30739] - Immunofluorescence staining of SON in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-30739 (dilution ratio 1:100) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202511545686.png "Immunocytochemistry/ Immunofluorescence: SON Antibody - BSA Free [NBP3-30739] - Immunofluorescence staining of SON in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-30739 (dilution ratio 1:100) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SON Antibody - BSA Free [NBP3-30739] - Immunochemical staining of human SON in human prostate with NBP3-30739 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202514492369.png)
![Immunohistochemistry-Paraffin: SON Antibody - BSA Free [NBP3-30739] - Immunochemical staining of human SON in human small intestine with NBP3-30739 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-30739_rabbit-son-pab-235202514371322.png)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0002.jpg "Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP2-87080] - Rabbit Anti-BRD7 antibody. Paraffin Embedded Tissue: Human Lung. Cellular Data: alveolar cell. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP2-87080-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - Host: Rabbit. Target Name: BRD7. Sample Type: Jurkat. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%BRD7](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0003.jpg)
![Western Blot: ELAC2 Antibody [NBP2-97772] - Anti-ELAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/ELAC2-Antibody-Western-Blot-NBP2-97772-img0004.jpg "Western Blot: ELAC2 Antibody [NBP2-97772] - Anti-ELAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa")
![Immunoprecipitation: ELAC2 Antibody [NBP2-97772] - ELAC2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-ELAC2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ELAC2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunoprecipitation-NBP2-97772-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ELAC2 Antibody [NBP2-97772] - Immunofluorescence staining of ELAC2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97772 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97772_rabbit-polyclonal-elac2-antibody-235202521451778.png)
![Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-288202414191513.jpg "Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Immunocytochemistry analysis of HeLa cells labeling VE-Cadherin with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-29202410234272.jpg)
![Flow Cytometry: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Flow cytometric analysis of EA.hy926 cells labeling VE-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33088, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-2920241645321.jpg)
![Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-278202416285235.jpg "Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-29820241435241.jpg)
![Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-278202415212437.jpg "Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32233) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-29820241492664.jpg)
![Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-T1-Antibody-Immunohistochemistry-Paraffin-NBP3-06557-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-T1-Antibody-Immunohistochemistry-Paraffin-NBP3-06557-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin T1 Antibody [NBP3-06557] - Staining of CCNT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06557 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06557_rabbit-polyclonal-cyclin-t1-antibody-23520252135186.png)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)