| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59611-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50055SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59614-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54460-100ug | |||
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP3-00016-img0005.jpg "Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human malignant melanoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0003.jpg)
![Immunoprecipitation: FABP7/B-FABP Antibody [NBP3-00016] - Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-FABP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FABP7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunoprecipitation-NBP3-00016-img0006.jpg)
![Western Blot: ID3 Antibody (2B11) [NB500-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-2B11-Western-Blot-NB500-112-img0003.jpg "Western Blot: ID3 Antibody (2B11) [NB500-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ID3 Antibody (2B11) [NB500-112] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Id3 protein stained by Id3 antibody [2B11] diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/ID3-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NB500-112-img0001.jpg)
![Western Blot: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Western-Blot-NBP2-59610-img0006.jpg "Western Blot: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1468).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Immunohistochemistry-Paraffin-NBP2-59610-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1468) [NBP2-59610] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Antibody (MCM7/1468) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1468-Protein-Array-NBP2-59610-img0005.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Western-Blot-NBP2-19526-img0002.jpg "Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526] - Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunohistochemistry-Paraffin-NBP2-19526-img0003.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIB antibody [N1C2] (NBP2-19526) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19526_rabbit-polyclonal-nfib-antibody-9102024209148.jpg)
![NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] (NBP2-19526) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-19526_rabbit-polyclonal-nfib-antibody-immunohistochemistry-2611202518345220.jpg)
![Western Blot: CDK5RAP2 Antibody [NB100-767] - Detection of Human CDK5RAP2 on HeLa whole cell lysate using NB100-767. CDK5RAP2 was also immunoprecipitated using another rabbit anti-CDK5RAP2 antibody.](http://images.novusbio.com/fullsize/CDK5RAP2-Antibody-Western-Blot-NB100-767-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CDK5RAP2 Antibody [NB100-767] - Detection of Human CDK5RAP2 on HeLa whole cell lysate using NB100-767. CDK5RAP2 was also immunoprecipitated using another rabbit anti-CDK5RAP2 antibody.")
![Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055] - Analysis of a region of cerebral cortex from an Alzheimer's disease (AD) patient stained with NBP2-50055, the signal detected with HRP and DAB. Senile plaques are labeled SP. The region of the lowest of the three plaques is shown in the inset stained with the fluorescent dye thioflavin-S. This dye binds to not only the senile plaque but also a neurofibrillary tangle (NFT), the other pathological hallmark of AD, which does not contain ABeta.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-AB9-NBP2-50055-img0005.jpg "Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055] - Analysis of a region of cerebral cortex from an Alzheimer's disease (AD) patient stained with NBP2-50055, the signal detected with HRP and DAB. Senile plaques are labeled SP. The region of the lowest of the three plaques is shown in the inset stained with the fluorescent dye thioflavin-S. This dye binds to not only the senile plaque but also a neurofibrillary tangle (NFT), the other pathological hallmark of AD, which does not contain ABeta.")
![Western Blot: beta Amyloid Antibody (AB9) [NBP2-50055] - Amyloid beta peptide blotted with NBP2-50055. NBP2-50055 recognizes amyloid beta peptide running at a molecular weight of 5 kDa and amyloid beta aggregates.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-AB9-Western-Blot-NBP2-50055-img0004.jpg)
![Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Western-Blot-NBP2-59613-img0004.jpg "Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Immunohistochemistry-Paraffin-NBP2-59613-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Protein-Array-NBP2-59613-img0005.jpg)
![Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-31496-img0004.jpg "Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Sample: Paraffin-embedded mouse ovary. Septin-2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0003.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-2 antibody [N1N3] (NBP1-31496) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31496_rabbit-polyclonal-septin-2-antibody-western-blot-141120251839106.jpg)
![Western Blot: MCM7 Antibody (SPM379) [NBP2-53219] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (SPM379).](http://images.novusbio.com/fullsize/MCM7-Antibody-SPM379-Western-Blot-NBP2-53219-img0002.jpg "Western Blot: MCM7 Antibody (SPM379) [NBP2-53219] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (SPM379).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (SPM379).](http://images.novusbio.com/fullsize/MCM7-Antibody-SPM379-Immunohistochemistry-Paraffin-NBP2-53219-img0001.jpg)
![Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241715429.jpg "Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800")
![Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody (8H4) [NBP3-43032] - Immunofluorescence analysis of U2OS cells using Septin-2 antibody (FNab07722). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241723695.jpg)
![Western Blot: Septin-2 Antibody (8H4) [NBP3-43032] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (Septin-2 Antibody) at 1:8000.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241734110.jpg)