| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05378-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37326-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48223 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48202 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54310-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-518SS | |||
![Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0002.jpg "Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ATF5 Antibody [NBP1-51908] - Analysis of ATF5 antibody in human heart lysate (RIPA buffer, 35 ug total loaded per lane) using NBP1-51908 at 1.5 ug/ml. Primary incubated for 1 hour. ATF5 antibody detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0001.jpg)
![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0004.jpg "Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.")
![Flow Cytometry: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Flow-Cytometry-NBP2-37326-img0005.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0002.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0003.jpg)
![ELISA: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-ELISA-NBP2-37326-img0001.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (31G7) - Azide and BSA Free [NBP2-47740] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47740-img0002.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (31G7) - Azide and BSA Free [NBP2-47740] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).")
![Protein Array: EGFR Antibody (31G7) - Azide and BSA Free [NBP2-47740] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (31G7) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Azide-and-BSA-Free-Protein-Array-NBP2-47740-img0001.jpg)
![Western Blot: EGFR Antibody (GFR/1667) [NBP2-53346] - Western Blot Analysis of A431 cell lysate using EGFR antibody (GFR/1667).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1667-Western-Blot-NBP2-53346-img0006.jpg "Western Blot: EGFR Antibody (GFR/1667) [NBP2-53346] - Western Blot Analysis of A431 cell lysate using EGFR antibody (GFR/1667).")
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR/1667) [NBP2-53346] - Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Antibody (GFR/1667).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1667-Immunohistochemistry-Paraffin-NBP2-53346-img0005.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR/1667) [NBP2-53346] - Formalin-fixed, paraffin-embedded human Lung SCC stained with EGFR Monoclonal Antibody (GFR/1667).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1667-Immunohistochemistry-Paraffin-NBP2-53346-img0001.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR/1667) [NBP2-53346] - Formalin-fixed, paraffin-embedded human Bladder stained with EGFR Antibody (GFR/1667).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1667-Immunohistochemistry-Paraffin-NBP2-53346-img0004.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-518] - Analysis of NSP 5 alpha 3 on HeLa whole cell extract.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-518-img0002.jpg "Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-518] - Analysis of NSP 5 alpha 3 on HeLa whole cell extract.")
![Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Western-Blot-NBP3-15178-img0004.jpg "Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody (3E6) [NBP3-15178] - Immunofluorescence staining of Hela cells with at CDC25C antibody 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunocytochemistry-Immunofluorescence-NBP3-15178-img0001.jpg)
![Immunohistochemistry: CDC25C Antibody (3E6) [NBP3-15178] - IHC image of CDC25C antibody diluted at 1:165 and staining in paraffin-embedded human glioma cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunohistochemistry-NBP3-15178-img0003.jpg)
![Immunoprecipitation: CDC25C Antibody (3E6) [NBP3-15178] - Immunoprecipitating CDC25C in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of CDC25C antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CDC25C antibody (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunoprecipitation-NBP3-15178-img0002.jpg)
![Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EML4-Antibody-Western-Blot-NBP1-05973-img0004.jpg "Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EML4 used at a dilution of1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-05973-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EML4 Antibody [NBP1-05973] - Detection of human EML4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EML4 antibody NBP1-05973 (lot NBP1-05973-2) used for IP at 6 ug per reaction. EML4 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05973-1) and rabbit anti-EML4 antibody NBP1-05974. For blotting immunoprecipitated EML4, NBP1-05973 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunoprecipitation-NBP1-05973-img0006.jpg)