| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05378-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38226-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92902-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37968-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35501-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61735-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2721-0.025mg | |||
![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415111899.jpg "Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: CIRBP Antibody [NBP3-38226] - Western blot analysis of lysates from Rat testis, using CIRBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121323.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human placenta using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121388.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Rat brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202414163069.jpg)
![Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP2-92902-img0005.jpg "Western Blot: Cyclin C Antibody [NBP2-92902] - Analysis of extracts of various cell lines, using Cyclin C at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded rat brain using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human colon carcinoma using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP2-92902] - Paraffin-embedded human lung cancer using Cyclin C .](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-92902-img0003.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411545578.jpg "Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: Cullin 1 Antibody [NBP3-37968] - Western blot analysis of various lysates, using Cullin 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241243916.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411572689.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241252829.jpg)
![Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35501_rabbit-cdc23-pab-10122024120617.jpg "Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35501_rabbit-cdc23-pab-101220241233855.jpg)
![Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35501_rabbit-cdc23-pab-1012202411531049.jpg)
![Western Blot: Cdc23 Antibody [NBP3-35501] - Western blot analysis of various lysates using Cdc23 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35501_rabbit-cdc23-pab-101220241252873.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts of various cell lines, using p18INK4c/CDKN2C Rabbit mAb (NBP3-16695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/p18INK4c-CDKN2C-Antibody-1Q10Q4-Western-Blot-NBP3-16695-img0002.jpg "Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts of various cell lines, using p18INK4c/CDKN2C Rabbit mAb (NBP3-16695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Immunofluorescence analysis of HeLa cells using p18INK4c/CDKN2C Rabbit mAb (NBP3-16695) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/p18INK4c-CDKN2C-Antibody-1Q10Q4-Immunocytochemistry-Immunofluorescence-NBP3-16695-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts from wild type(WT) and CDKN2C knockout (KO) 293T cells, using CDKN2C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16695_rabbit-p18ink4c-cdkn2c-mab-1q10q4-822025209835.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts of HepG2 cells, using CDKN2C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16695_rabbit-p18ink4c-cdkn2c-mab-1q10q4-822025208450.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0004.jpg "Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)")
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0005.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded colon cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0002.jpg)
![ELISA: CIRBP Antibody (6E12D5) [NBP2-61735] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-ELISA-NBP2-61735-img0001.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0004.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.")
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0001.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0002.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0003.jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Western-Blot-NBP2-73184-img0001.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73184-img0002.jpg)
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)