| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03981SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06496-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12710-50ul | |||
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981] - Western blot analysis of extracts from HepG2 cells using NBP2-03981](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-03981-img0001.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981] - Western blot analysis of extracts from HepG2 cells using NBP2-03981")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981] - mmunofluorescence staining of methanol-fixed Hela cells using NBP2-03981](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-03981-img0002.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-03981] - Western blotting analysis using NBP2-03981.](http://images.novusbio.com/fullsize/nbp2-03981_rabbit-polyclonal-histone-deacetylase-4-hdac4-antibody-103202523472636.jpg)
![Western Blot: GFI1B Antibody [NBP2-84040] - WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/GFI1B-Antibody-Western-Blot-NBP2-84040-img0003.jpg "Western Blot: GFI1B Antibody [NBP2-84040] - WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate")
![Immunohistochemistry-Paraffin: GFI1B Antibody [NBP2-84040] - Rabbit Anti-GFI1B Antibody. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule and renal corpuscle. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/GFI1B-Antibody-Immunohistochemistry-Paraffin-NBP2-84040-img0002.jpg)
![Western Blot: GFI1B Antibody [NBP2-84040] - Host: Rabbit. Target: GFI1B. Positive control (+): Mouse testis (M-TE). Negative control (-): Rat liver (R-LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/GFI1B-Antibody-Western-Blot-NBP2-84040-img0004.jpg)
![Immunohistochemistry-Paraffin: GFI1B Antibody [NBP2-84040] - Rabbit Anti-GFI1B Antibody. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/GFI1B-Antibody-Immunohistochemistry-Paraffin-NBP2-84040-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0004.jpg "Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in cynomolgus heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in human colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0002.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0003.jpg "Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with testis at 5.2 ug/ml.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-NLS7979-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NLS7979] - Analysis of anti-CDC7 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/CDC7-Antibody-Immunohistochemistry-Paraffin-NLS7979-img0002.jpg)
![Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-288202412493422.jpg "Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33049) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-298202414304128.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunocytochemistry analysis of SiHa cells labeling TRF-2 with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-29202410334036.jpg)
![Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-278202416331292.jpg "Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-298202414304159.jpg)
![Flow Cytometry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Flow cytometric analysis of K-562 cells labeling E2F-4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-29202416372181.jpg)
![Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Western-Blot-NBP3-06496-img0001.jpg "Western Blot: CEND1 Antibody [NBP3-06496] - Lane A: SH-SY5Y Whole Cell LysateLane B: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size: kDa")
![Immunohistochemistry-Paraffin: CEND1 Antibody [NBP3-06496] - Staining of human CEND1 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunohistochemistry-Paraffin-NBP3-06496-img0002.jpg)
![Immunoprecipitation: CEND1 Antibody [NBP3-06496] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-CEND1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CEND1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/CEND1-Antibody-Immunoprecipitation-NBP3-06496-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: HYLS1 Antibody [NBP3-12710] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HYLS1-Antibody-Western-Blot-NBP3-12710-img0001.jpg "Western Blot: HYLS1 Antibody [NBP3-12710] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HYLS1 Antibody [NBP3-12710] - Staining of human HYLS1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/HYLS1-Antibody-Immunohistochemistry-Paraffin-NBP3-12710-img0002.jpg)
![Immunoprecipitation: HYLS1 Antibody [NBP3-12710] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-HYLS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HYLS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDa](http://images.novusbio.com/fullsize/HYLS1-Antibody-Immunoprecipitation-NBP3-12710-img0003.jpg)
![Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-278202416285255.jpg "Western Blot: CLIC4 Antibody (JE65-09) [NBP3-32192] - Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: RAW264.7 cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Human kidney tissue lysate (40 ug/Lane) Lane 8: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CLIC4 Antibody (JE65-09) [NBP3-32192] - Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody (NBP3-32192) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-2920241114835.jpg)
![Flow Cytometry: CLIC4 Antibody (JE65-09) [NBP3-32192] - Flow cytometric analysis of HeLa cells labeling CLIC4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32192, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32192_rabbit-clic4-mab-je65-09-29202416372180.jpg)