| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06557-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06477-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81058-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26428-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26502-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27825-50ul | |||
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of various lysates, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-912202414163021.jpg "Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of various lysates, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (6O8F8) [NBP3-33430] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-52202511411411.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody (6O8F8) [Cdk5] - Western blot analysis of lysates from wild type(WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33430_rabbit-cdk5-mab-6o8f8-52202517352724.jpg)
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-T1-Antibody-Immunohistochemistry-Paraffin-NBP3-06557-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP3-06557] - Staining of human CCNT1 in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-T1-Antibody-Immunohistochemistry-Paraffin-NBP3-06557-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin T1 Antibody [NBP3-06557] - Staining of CCNT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06557 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06557_rabbit-polyclonal-cyclin-t1-antibody-23520252135186.png)
![Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Western-Blot-NBP3-06477-img0001.jpg "Western Blot: MNAT1 Antibody [NBP3-06477] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa")
![Immunoprecipitation: MNAT1 Antibody [NBP3-06477] - Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-MNAT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MNAT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :35 kDa](http://images.novusbio.com/fullsize/MNAT1-Antibody-Immunoprecipitation-NBP3-06477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MNAT1 Antibody [NBP3-06477] - Staining of MNAT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06477 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06477_rabbit-polyclonal-mnat1-antibody-235202511444422.png)
![Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Western-Blot-NBP2-81058-img0001.jpg "Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.")
![Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81058] - Immunohistochemical staining of rabbit brain using [NBP2-81058]. Formalin fixed rabbit brain slices were were stained with NBP2-81058 at 3 ug/ml.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Immunohistochemistry-NBP2-81058-img0002.jpg)
![Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-262202413221513.jpg "Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
![Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not). All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 34 KDaObserved band size: 34 KDa](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241140949.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241312220.jpg)
![Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-282202411413611.jpg "Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa")
![Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-28220241312218.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Western Blot: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Western blot detection of MCM2 [p Ser40] in Hela, A549, MCF-7, 293T, C2C12 cell lysates using MCM2 [p Ser40] Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-p-Ser40-Antibody-S03-1B3-Western-Blot-NBP3-14952-img0001.jpg "Western Blot: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Western blot detection of MCM2 [p Ser40] in Hela, A549, MCF-7, 293T, C2C12 cell lysates using MCM2 [p Ser40] Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunohistochemistry-Paraffin: MCM2 [p Ser40] Antibody (S03-1B3) [NBP3-14952] - Immunohistochemistry of MCM2 [p Ser40] in paraffin-embedded Human tonsil using MCM2 [p Ser40] Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/MCM2-p-Ser40-Antibody-S03-1B3-Immunohistochemistry-Paraffin-NBP3-14952-img0002.jpg)
![Western Blot: MCM2 Antibody (S08-9H4) [NBP3-14951] - Western blot detection of MCM2 in K562, C6, 3T3, Hela cell lysates using MCM2 Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.](http://images.novusbio.com/fullsize/MCM2-Antibody-S08-9H4-Western-Blot-NBP3-14951-img0001.jpg "Western Blot: MCM2 Antibody (S08-9H4) [NBP3-14951] - Western blot detection of MCM2 in K562, C6, 3T3, Hela cell lysates using MCM2 Rabbit mAb (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S08-9H4) [NBP3-14951] - Immunofluorescence of MCM2 (green) in Hela cells using NBP3-14951 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14951_rabbit-mcm2-mab-s08-9h4-113202515145410.jpg)
![Western Blot: MCM2 [p Ser 27] Antibody (SR1107) [NBP3-27825] - Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate.](http://images.novusbio.com/fullsize/nbp3-27825_rabbit-mcm2-p-ser-27-mab-sr1107-75202410133433.jpg "Western Blot: MCM2 [p Ser 27] Antibody (SR1107) [NBP3-27825] - Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate.")