| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75903SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
![Western Blot: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Jurkat (A) (0.03µg/ml) and Molt-4 (B) (0.03µg/ml) cells lysates, and Jurkat (C) (0.01µg/ml) cells nuclear lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with NBP3-43576 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-western-blot-2220260322010.png "Western Blot: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Jurkat (A) (0.03µg/ml) and Molt-4 (B) (0.03µg/ml) cells lysates, and Jurkat (C) (0.01µg/ml) cells nuclear lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with NBP3-43576 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.")
![Flow Cytometry: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or tNBP3-43576 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-flow-cytometry-222026033316.png)
![Immunocytochemistry/ Immunofluorescence: TdT Antibody (32D5) - Chimeric - BSA Free [NBP3-43576] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on plus coverslips, permeabilized with 0.15% Triton, stained with NBP3-43576 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43576, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43576_rabbit-tdt-mab-32d5-chimeric-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2220260322023.png)
![Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-Immunohistochemistry-NBP2-75903-img0002.jpg "Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.")
![ELISA: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (NBP2-75903).](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-ELISA-NBP2-75903-img0001.jpg)
![Immunohistochemistry-Paraffin: TSH R Antibody (SPM223) [NBP2-54569] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRA Monoclonal Antibody (SPM223).](http://images.novusbio.com/fullsize/TSH-R-Antibody-SPM223-Immunohistochemistry-Paraffin-NBP2-54569-img0001.jpg "Immunohistochemistry-Paraffin: TSH R Antibody (SPM223) [NBP2-54569] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with TSHRA Monoclonal Antibody (SPM223).")
![Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/E2F8-Antibody-Western-Blot-NBP1-52650-img0006.jpg "Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody NBP1-52650(lot NBP1-52650-3) used for IP at 6 ug per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-52650-2). For blotting immunoprecipitated E2F8, NBP1-52650 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/E2F8-Antibody-Immunoprecipitation-NBP1-52650-img0007.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NBP2-16786-img0005.jpg "Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/HCFC1-Antibody-Western-Blot-NBP2-16786-img0001.jpg)
![Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term (NBP2-16786) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16786_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-western-blot-2611202518454019.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202414471591.jpg "Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202415253332.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (3A5) [NBP3-26692] - Staining of Hela Cells with Cyclin E1 Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-282202412493621.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0002.jpg "Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.")
![Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0001.jpg)
