| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35562-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05613-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92865-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1312-0.025ml | |||
![Western Blot: PCNP Antibody [NBP3-35562] - Western blot analysis of various lysates using PCNP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35562_rabbit-pcnp-pab-912202415121384.jpg "Western Blot: PCNP Antibody [NBP3-35562] - Western blot analysis of various lysates using PCNP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NBP3-05613-img0001.jpg "Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: DP1 Antibody [NBP2-92865] - Paraffin-embedded rat testis using DP1 .](http://images.novusbio.com/fullsize/DP1-Antibody-Immunohistochemistry-Paraffin-NBP2-92865-img0001.jpg "Immunohistochemistry-Paraffin: DP1 Antibody [NBP2-92865] - Paraffin-embedded rat testis using DP1 .")
![Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/p53-[p-Ser392]-Antibody-Western-Blot-NB200-156-img0001.jpg "Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: TRF-1 Antibody (JE59-70) [NBP3-33048] - Western blot analysis of TRF-1 on different lysates with Rabbit anti-TRF-1 antibody (NBP3-33048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: U-2 OS cell lysate Lane 4: MCF7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33048) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33048_rabbit-trf-1-mab-je59-70-28820241249340.jpg "Western Blot: TRF-1 Antibody (JE59-70) [NBP3-33048] - Western blot analysis of TRF-1 on different lysates with Rabbit anti-TRF-1 antibody (NBP3-33048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: U-2 OS cell lysate Lane 4: MCF7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33048) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ki67/MKI67 Antibody (PD00-10) [NBP3-32453] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Ki67/MKI67 antibody (NBP3-32453) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32453) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32453_mouse-ki67-mki67-mab-pd00-10-298202414333629.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (PD00-10) [NBP3-32453] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Ki67/MKI67 antibody (NBP3-32453) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32453) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32812_rabbit-tsc2-p-ser939-mab-je54-06-28820241244294.jpg "Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: p19 INK4d Antibody [NBP2-19651] - Sample (50 ug of whole cell lysate) A: Rat bone marrow, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/p19-INK4d-Antibody-Western-Blot-NBP2-19651-img0001.jpg "Western Blot: p19 INK4d Antibody [NBP2-19651] - Sample (50 ug of whole cell lysate) A: Rat bone marrow, 12% SDS PAGE gel, diluted at 1:500.")
![Western Blot: CtIP Antibody [NBP1-33098] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [C1C3] (NBP1-33098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33098_rabbit-polyclonal-ctip-antibody-91020242075926.jpg "Western Blot: CtIP Antibody [NBP1-33098] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [C1C3] (NBP1-33098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cdk3 Antibody [NBP1-32230] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Cdk3 antibody diluted at 1:1000](http://images.novusbio.com/fullsize/Cdk3-Antibody-Western-Blot-NBP1-32230-img0001.jpg "Western Blot: Cdk3 Antibody [NBP1-32230] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Cdk3 antibody diluted at 1:1000")