| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94045-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38164-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92653-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93042-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61719-0.025ml | |||
![Western Blot: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of extracts of various cell lines, using SCP2/SYCP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/SCP2-SYCP2-Antibody-Western-Blot-NBP2-94045-img0002.jpg "Western Blot: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of extracts of various cell lines, using SCP2/SYCP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of C6 cells using SCP2/SYCP2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SCP2-SYCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94045-img0001.jpg)
![Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411421066.jpg "Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP3-38164] - Immunofluorescence analysis of HeLa cells using FZR1/CDH1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411353070.jpg)
![Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Western-Blot-NBP2-92653-img0003.jpg "Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Centrin-2-Antibody-Knockout-Validated-NBP2-92653-img0005.jpg)
![Western Blot: SETD8 Antibody [NBP2-93042] - Analysis of extracts of various cell lines, using SETD8 . Exposure time: 60s.](http://images.novusbio.com/fullsize/SETD8-Antibody-Western-Blot-NBP2-93042-img0002.jpg "Western Blot: SETD8 Antibody [NBP2-93042] - Analysis of extracts of various cell lines, using SETD8 . Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: SETD8 Antibody [NBP2-93042] - Analysis of MCF-7 cells using SETD8 .](http://images.novusbio.com/fullsize/SETD8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93042-img0001.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0003.jpg "Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)")
![Flow Cytometry: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Flow-Cytometry-NBP2-61719-img0002.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0004.jpg)
![ELISA: Bub1 Antibody (1A11C7) [NBP2-61719] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-ELISA-NBP2-61719-img0001.jpg)
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)
![Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Western-Blot-NB100-2364-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2364-img0002.jpg)