| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30536-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90024-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73552 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76698-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38146-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35246-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95103-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30536-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.")
![Immunohistochemistry-Paraffin: B7-H4 Antibody [NBP2-30536] - Staining of human breast shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-30536-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody [NBP1-90024] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.](http://images.novusbio.com/fullsize/Chk2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90024-img0006.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody [NBP1-90024] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: Chk2 Antibody [NBP1-90024] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-Immunohistochemistry-Paraffin-NBP1-90024-img0003.jpg)
![Western Blot: PPM1G Antibody (1C12) [NBP2-03463] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/PPM1G-Antibody-1C12-Western-Blot-NBP2-03463-img0003.jpg "Western Blot: PPM1G Antibody (1C12) [NBP2-03463] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: PPM1G Antibody (1C12) [NBP2-03463] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G.](http://images.novusbio.com/fullsize/PPM1G-Antibody-1C12-Immunocytochemistry-Immunofluorescence-NBP2-03463-img0002.jpg)
![Western Blot: PPM1G Antibody (1C12) [NBP2-03463] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G.](http://images.novusbio.com/fullsize/PPM1G-Antibody-1C12-Western-Blot-NBP2-03463-img0001.jpg)
![Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76698_rabbit-polyclonal-il-27-antibody-2711202420212370.jpg)
![Immunocytochemistry/ Immunofluorescence: HUS1 Antibody [NBP3-38146] - Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38146_rabbit-hus1-pab-1012202411381226.jpg "Immunocytochemistry/ Immunofluorescence: HUS1 Antibody [NBP3-38146] - Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: HUS1 Antibody [NBP3-38146] - Western blot analysis of various lysates using HUS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38146_rabbit-hus1-pab-1012202411433416.jpg)
![Immunoprecipitation: HUS1 Antibody [NBP3-38146] - Immunoprecipitation analysis of 200 ug extracts of 293T cells using HUS1 antibody. Western blot was performed from the immunoprecipitate using HUS1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38146_rabbit-hus1-pab-101220241134334.jpg)
![Immunocytochemistry/ Immunofluorescence: HUS1 Antibody [NBP3-38146] - Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38146_rabbit-hus1-pab-101220241136031.jpg)
![Western Blot: Topoisomerase I Antibody [NBP3-35246] - Western blot analysis of various lysates using Topoisomerase I(TOP1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35246_rabbit-topoisomerase-i-pab-101220241224150.jpg "Western Blot: Topoisomerase I Antibody [NBP3-35246] - Western blot analysis of various lysates using Topoisomerase I(TOP1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunoprecipitation: Topoisomerase I Antibody [NBP3-35246] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug Topoisomerase I(Topoisomerase I(TOP1)) antibody. Western blot was performed from the immunoprecipitate using Topoisomerase I(Topoisomerase I(TOP1)) antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35246_rabbit-topoisomerase-i-pab-1012202411592891.jpg)
![Immunohistochemistry: Topoisomerase I Antibody [NBP3-35246] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Topoisomerase I(TOP1) Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35246_rabbit-topoisomerase-i-pab-101220241233822.jpg)
![Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of K-562 cells, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NBP2-95103-img0004.jpg "Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of K-562 cells, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Mre11 Antibody [NBP2-95103] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Mre11 Rabbit pAb (NBP2-95103) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NBP2-95103-img0003.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody [NBP2-95103] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Mre11 Rabbit pAb (NBP2-95103) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NBP2-95103-img0002.jpg)
![Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of various cell lines, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Mre11 Antibody-Western Blot-NBP2-95103-img0005.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Western-Blot-NBP1-71840-img0007.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71840] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- EBP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-NBP1-71840-img0008.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-71839-img0004.jpg "Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).")
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394115..jpg)
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394246..jpg)
![TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41217_mouse-monoclonal-trf-1-antibody-4e4-immunocytochemistry-immunofluorescence-261120251838405.jpg "TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.")
