| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72565 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35395-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38567-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99631-50ul | |||
![Western Blot: RFC2 Antibody [NBP1-32253] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/RFC2-Antibody-Western-Blot-NBP1-32253-img0003.jpg "Western Blot: RFC2 Antibody [NBP1-32253] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-32253] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RFC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32253-img0002.jpg)
![Western Blot: RFC2 Antibody [NBP1-32253] - A: A431 B: H1299 C: Hela 10% SDS PAGE; antibody diluted at 1:1000](http://images.novusbio.com/fullsize/RFC2-Antibody-Western-Blot-NBP1-32253-img0001.jpg)
![Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.](http://images.novusbio.com/fullsize/Artemis-Antibody-Western-Blot-NBP1-77975-img0002.jpg "Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.")
![Immunohistochemistry: Artemis Antibody [NBP1-77975] - Used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Artemis-Antibody-Immunohistochemistry-NBP1-77975-img0003.jpg)
![Western Blot: Artemis Antibody [NBP2-44276] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells prepared using NETN lysis buffer. NBP2-44276 was also immunoprecipitated by another rabbit anti-Artemis antibody.](http://images.novusbio.com/fullsize/Artemis-Antibody-Western-Blot-NBP2-44276-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Artemis Antibody [NBP2-44276] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells prepared using NETN lysis buffer. NBP2-44276 was also immunoprecipitated by another rabbit anti-Artemis antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Artemis Antibody [NBP2-44276] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH3T3, C2C12, F9, and Renca cells prepared using NETN lysis buffer.](http://images.novusbio.com/fullsize/Artemis-Antibody-Western-Blot-NBP2-44276-img0005.jpg)
![Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13076-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Chk2 [p Thr68] Antibody [NBP3-13076] - Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0003.jpg)
![Western Blot: Mad2L1 Antibody (4D2) [NBP2-02103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mad2L1.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-4D2-Western-Blot-NBP2-02103-img0001.jpg "Western Blot: Mad2L1 Antibody (4D2) [NBP2-02103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mad2L1.")
![Immunocytochemistry/Immunofluorescence: Mad2L1 Antibody (4D2) [NBP2-02103] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mad2L1.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP2-02103-img0002.jpg)
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: TUSC4 Antibody [NBP3-35395] - Western blot analysis of various lysates using TUSC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35395_rabbit-tusc4-pab-912202415171143.jpg "Western Blot: TUSC4 Antibody [NBP3-35395] - Western blot analysis of various lysates using TUSC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: TUSC4 Antibody [NBP3-35395] - Immunofluorescence analysis of HeLa cells using TUSC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35395_rabbit-tusc4-pab-912202415121396.jpg)
![Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415171153.jpg "Western Blot: BCCIP Antibody [NBP3-38567] - Western blot analysis of various lysates using BCCIP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunohistochemistry: BCCIP Antibody [NBP3-38567] - Immunohistochemistry analysis of paraffin-embedded Human breast using BCCIP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38567_rabbit-bccip-pab-912202415175244.jpg)
![Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-NBP1-02798-img0003.jpg "Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.")
![Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Immunohistochemistry-Paraffin-NBP1-02798-img0001.jpg)
![Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-298202414262624.jpg "Immunohistochemistry: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32755) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Chk1 [p Ser317] Antibody (PSH03-84) [NBP3-32755] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32755) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32755_rabbit-chk1-p-ser317-mab-psh03-84-392024848501.jpg)
![Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human kidney with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-136202422345047.png "Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human kidney with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - NBP3-26072 at 1:500 dilution. Lane A: THP-1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-136202422345068.png)
![Immunohistochemistry-Paraffin: RAD9B Antibody - Azide and BSA Free [NBP3-26072] - Immunochemical staining of human RAD9B in human skeletal muscle with NBP3-26072 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26072_rabbit-rad9b-pab-azide-and-bsa-free-1362024223417.png)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-99631-img0005.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-99631-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99631 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99631_rabbit-polyclonal-14-3-3-sigma-stratifin-antibody-23520251433152.png)