| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71027 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70979 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71239 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg)
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg)
![HRAS Antibody (OTI1D9)[NBP2-45745]Simple Western� analysis of endogenous protein MCAM from HELA lysates (0.1 mg/mL) using CD146 (MCAM) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45745_mouse-hras-mab-oti1d9-simple-western-131120251236222.jpg%253fstore=america&image-type=image)
![Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000](http://images.novusbio.com/fullsize/IL6-Antibody-3G9-Western-Blot-NBP1-47810-img0002.jpg "Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP1-47810-img0019.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0034.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0021.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0025.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0027.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0028.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0029.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0030.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720249362622.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003225.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003219.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003236.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044651.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024936263.jpg)
![Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044633.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024959423.jpg)
![Western Blot: ID3 Antibody (OTI8B3) [NBP2-02136] - Analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1), human ID3 plasmid (RC200583, lane 2), mouse ID3 plasmid (MR227292, lane 3) using anti-ID3 antibody at 1:500.](http://images.novusbio.com/fullsize/antibody/nbp2-02136_mouse-id3-mab-oti8b3-western-blot-22102024114327..jpg "Western Blot: ID3 Antibody (OTI8B3) [NBP2-02136] - Analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1), human ID3 plasmid (RC200583, lane 2), mouse ID3 plasmid (MR227292, lane 3) using anti-ID3 antibody at 1:500.")
![Immunocytochemistry/Immunofluorescence: ID3 Antibody (OTI8B3) [NBP2-02136] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunocytochemistry-Immunofluorescence-NBP2-02136-img0011.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human thyroid tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0017.jpg)
![Flow Cytometry: ID3 Antibody (OTI8B3) [NBP2-02136] - Analysis of Jurkat cells, using anti-ID3 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ID3-Antibody-OTI8B3-Flow-Cytometry-NBP2-02136-img0019.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human colon tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0012.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human Kidney tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0013.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human liver tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0014.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human Ovary tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0015.jpg)
![Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human prostate tissue using anti-ID3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Immunohistochemistry-Paraffin-NBP2-02136-img0016.jpg)
![Flow Cytometry: ID3 Antibody (8B3) [NBP2-02136] - Analysis of Hela cells, using anti-ID3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ID3-Antibody-8B3-Flow-Cytometry-NBP2-02136-img0010.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0007.jpg "Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0006.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0002.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0005.jpg)
![Immunofluorescence: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunofluorescence-NBP2-45814-img0001.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HMGA1-Antibody-5J0E6-Western-Blot-NBP3-16379-img0005.jpg "Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511383742.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 across chromosome 12(upper panel) and the genomic region encompassing HNF1A, a representative gene enriched in HMGA1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251141141.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374182.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251255541.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511402740.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of lysates from Mouse testis, using HMGA1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215443.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 in the representative genomic region surrounding HNF1A gene.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374168.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374132.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215418.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511361253.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGA1 Antibody (5J0E6) [NBP3-16379] - Confocal imaging of U-2 OS cells using HMGA1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511411472.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215471.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374188.jpg)
![Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Western-Blot-NBP3-15469-img0007.jpg "Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of U-2 OS cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunocytochemistry-Immunofluorescence-NBP3-15469-img0005.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Mouse spinal cord using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0003.jpg)
![Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of various cell lines, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Western-Blot-NBP3-15469-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of C6 cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunocytochemistry-Immunofluorescence-NBP3-15469-img0004.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Rat brain using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0001.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Human esophageal using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0002.jpg)
![Immunoprecipitation: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of 300ug extracts of HeLa cells using 3ug KLF4 antibody (NBP3-15469). Western blot was performed from the immunoprecipitate using KLF4 antibody (NBP3-15469) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunoprecipitation-NBP3-15469-img0008.jpg)
![Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15469_rabbit-klf4-mab-7b7u1-52202517184556.jpg)
![Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [KLF4] - Confocal imaging of U-2 OS cells using KLF4 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15469_rabbit-klf4-mab-7b7u1-5220251718931.jpg)
![Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15469_rabbit-klf4-mab-7b7u1-5220251717281.jpg)
![Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15469_rabbit-klf4-mab-7b7u1-5220251718993.jpg)
![Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Human colon using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15469_rabbit-klf4-mab-7b7u1-52202517184562.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-6F9G8-Knockout-Validated-NBP3-15654-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311049.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517352755.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517304949.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-522025173458.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517344234.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311076.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517361268.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311081.jpg)
![Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Western-Blot-NBP2-70775-img0001.jpg "Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.")
![Immunocytochemistry/Immunofluorescence: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70775-img0003.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0009.jpg)
![Flow Cytometry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FRK antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70775-img0002.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0004.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0005.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0006.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0007.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0008.jpg)
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0010.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0002.jpg "Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71239-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0007.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0001.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0005.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0006.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)