| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89338-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB6000-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87245-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85731-25ul | |||
![Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1L4J1-Western-Blot-NBP3-15661-img0010.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![<b>Genetic Strategies Validation. </b>Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-western-blot-305202492857..jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517344271.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251730319.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251731552.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517361213.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517311090.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517352775.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Western-Blot-NBP2-75642-img0010.jpg "Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis")
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0004.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0009.jpg)
![Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Flow-Cytometry-NBP2-75642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0003.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0005.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0006.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0007.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0008.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa")
![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0011.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Western-Blot-MAB6000-img0002.jpg "Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.")
![Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Immunohistochemistry-MAB6000-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - An intracellular stain was performed on HeLa cells with Cyclin B1 Antibody [2061A] MAB6000F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0004.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - K562 human cell line was stained with Rabbit Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB6000, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0001.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NBP1-33105-img0007.jpg "Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MCM4 Antibody [NBP1-33105] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33105-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunohistochemistry-Paraffin-NBP1-33105-img0004.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - MCM4 antibody detects MCM4 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug NCI-H929 whole cell lysate/extract. 7.5 % SDS-PAGE. MCM4 antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/MCM4-Antibody-Western-Blot-NBP1-33105-img0006.jpg)
![Immunoprecipitation: MCM4 Antibody [NBP1-33105] - MCM4 antibody immunoprecipitates MCM4 protein in IP experiments. IP samples: Jurkat whole cell extract. A. 40 ug Jurkat whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM4 protein by 4 ug MCM4 antibody. 7.5 % SDS-PAGE. The immunoprecipitated MCM4 protein was detected by MCM4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/MCM4-Antibody-Immunoprecipitation-NBP1-33105-img0008.jpg)
![Immunohistochemistry: MCM4 Antibody [NBP1-33105] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using MCM4 antibody (NBP1-33105) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-91020242041276.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM4 Antibody [NBP1-33105] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-910202420134039.jpg)
![Western Blot: MCM4 Antibody [NBP1-33105] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-33105 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33105_rabbit-polyclonal-mcm4-antibody-91020242010442.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87245-img0014.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0018.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0009.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0008.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0011.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-PPM1G antibody NBP1-87245 (A) shows similar protein distribution across tissues to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0015.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human kidney.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0017.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0019.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody NBP1-85731 (A) shows similar protein distribution across tissues to independent antibody NBP1-85730 (B).](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-7 Antibody [NBP1-85731] - Staining of human cell line A-431 shows localization to nucleoli, plasma membrane & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85731-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0024.jpg)
![Western Blot: Septin-7 Antibody [NBP1-85731] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Western-Blot-NBP1-85731-img0018.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human testis shows moderate positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0021.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0022.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0023.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0025.jpg)
