| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93937-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35089-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50269-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04117-20ul | |||
![Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Western-Blot-H00004218-M02-img0003.jpg "Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Immunocytochemistry-Immunofluorescence-H00004218-M02-img0005.jpg)
![ELISA: RAB8A Antibody (3G1) [H00004218-M02] - Detection limit for recombinant GST tagged RAB8A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-ELISA-H00004218-M02-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42864-img0001.jpg "Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP2-42864-img0003.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP2-42864-img0002.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg)
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg)
![HRAS Antibody (OTI1D9)[NBP2-45745]Simple Western� analysis of endogenous protein MCAM from HELA lysates (0.1 mg/mL) using CD146 (MCAM) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45745_mouse-hras-mab-oti1d9-simple-western-131120251236222.jpg%253fstore=america&image-type=image)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Western Blot: PRL-2/PTP4A2 Antibody [NBP2-93937] - Analysis of extracts of various cell lines, using PRL-2/PTP4A2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PRL-2-PTP4A2-Antibody-Western-Blot-NBP2-93937-img0005.jpg "Western Blot: PRL-2/PTP4A2 Antibody [NBP2-93937] - Analysis of extracts of various cell lines, using PRL-2/PTP4A2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: PRL-2/PTP4A2 Antibody [NBP2-93937] - Analysis of C6 cells using PRL-2/PTP4A2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PRL-2-PTP4A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93937-img0001.jpg)
![Immunohistochemistry-Paraffin: PRL-2/PTP4A2 Antibody [NBP2-93937] - Rat kidney using PTP4A2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRL-2-PTP4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-93937-img0004.jpg)
![Immunohistochemistry-Paraffin: PRL-2/PTP4A2 Antibody [NBP2-93937] - Human colon using PTP4A2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRL-2-PTP4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-93937-img0002.jpg)
![Immunohistochemistry-Paraffin: PRL-2/PTP4A2 Antibody [NBP2-93937] - Mouse lung using PTP4A2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRL-2-PTP4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-93937-img0003.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-10122024121014.jpg "Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-101220241224118.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of lysates from wild type(WT) and RAP1A knockdown (KD) 293T cells, using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411552841.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411592829.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of various lysates using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411535893.jpg)
![Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Proximity-Ligation-Assay-H00005906-M01-img0002.jpg "Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: RAP1A Antibody (3E1) [H00005906-M01] - Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Sandwich-ELISA-H00005906-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-RAP1-4C8-1-Immunofluorescence-NBP2-50269-img0001.jpg "Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.")
![Western Blot: RAB8A Antibody (S06-8A7) [NBP3-19493] - Western blot detection of RAB8A in K562, C6, 3T3, Hela cell lysates using NBP3-19493 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.](http://images.novusbio.com/fullsize/RAB8A-Antibody-S06-8A7-Western-Blot-NBP3-19493-img0003.jpg "Western Blot: RAB8A Antibody (S06-8A7) [NBP3-19493] - Western blot detection of RAB8A in K562, C6, 3T3, Hela cell lysates using NBP3-19493 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.")
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody (S06-8A7) [NBP3-19493] - Immunofluorescence of RAB8A (green) in Hela cells using NBP3-19493 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/RAB8A-Antibody-S06-8A7-Immunocytochemistry-Immunofluorescence-NBP3-19493-img0001.jpg)
![Immunohistochemistry-Paraffin: RAB8A Antibody (S06-8A7) [NBP3-19493] - Immunohistochemistry of RAB8A in paraffin-embedded Human colon cancer tissue using NBP3-19493 at dilution 1/1](http://images.novusbio.com/fullsize/RAB8A-Antibody-S06-8A7-Immunohistochemistry-Paraffin-NBP3-19493-img0002.jpg)
![Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04117-img0003.jpg "Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric cancer using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0002.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody [NBP3-04519] - Analysis of U-2 OS cells using RAB8A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RAB8A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04519-img0001.jpg "Immunocytochemistry/Immunofluorescence: RAB8A Antibody [NBP3-04519] - Analysis of U-2 OS cells using RAB8A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: RAB8A Antibody - Azide and BSA Free [NBP3-04519] - Western blot analysis of extracts of C6 cells, using RAB8A antibody (A17369) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-04519_rabbit-polyclonal-rab8a-antibody-52202519302952.jpg)
![Western Blot: RAB8A Antibody - Azide and BSA Free [NBP3-04519] - Western blot analysis of extracts of various cell lines, using RAB8A antibody (A17369) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-04519_rabbit-polyclonal-rab8a-antibody-52202519302985.jpg)