| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71239 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56581-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72567 | |||
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0007.jpg "Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0006.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0002.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0005.jpg)
![Immunofluorescence: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunofluorescence-NBP2-45814-img0001.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0002.jpg "Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71239-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0007.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0001.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0005.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0006.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67378-img0008.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0007.jpg)
![Flow Cytometry: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Flow-Cytometry-NBP2-67378-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0006.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67373-img0006.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67373-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67373] - Analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67373-img0004.jpg)
![ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-244202310354.jpg "ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-2442023103658.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517344223.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517311071.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-5220251733559.jpg)
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-56581] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-56581-img0002.jpg "Western Blot: RABGAP1 Antibody [NBP2-56581] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: RABGAP1 Antibody [NBP2-56581] - Staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56581-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining in human thyroid gland and liver tissues using anti-RABGAP1 antibody. Corresponding RABGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0005.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0003.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-56581] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-56581-img0004.jpg)
![Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed.Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Western-Blot-NBP2-46060-img0012.jpg "Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed.Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)")
![Immunocytochemistry/Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human keratinocytes, PFA-fixed, permabilisation: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antiboy conjugated to Alexa Fluor 594. Red: MAD4 antibody, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Immunocytochemistry-Immunofluorescence-NBP2-46060-img0013.jpg)
![Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Immunohistochemistry-Paraffin-NBP2-46060-img0010.jpg)
![Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Immunohistochemistry-Paraffin-NBP2-46060-img0004.jpg)
![Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Immunohistochemistry-Paraffin-NBP2-46060-img0006.jpg)