| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92848-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92872-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98832-50ul | |||
![Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NB100-58788-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NB100-58788-img0003.jpg)
![Western Blot: TTK Antibody (4G9) [H00007272-M02] - TTK monoclonal antibody (M02), clone 4G9 Analysis of TTK expression in Jurkat.](http://images.novusbio.com/fullsize/TTK-Antibody-4G9-Western-Blot-H00007272-M02-img0004.jpg "Western Blot: TTK Antibody (4G9) [H00007272-M02] - TTK monoclonal antibody (M02), clone 4G9 Analysis of TTK expression in Jurkat.")
![ELISA: TTK Antibody (4G9) [H00007272-M02] - Detection limit for recombinant GST tagged TTK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TTK-Antibody-4G9-ELISA-H00007272-M02-img0005.jpg)
![Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-7W3E3-Western-Blot-NBP3-15821-img0004.jpg "Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded rat brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512435347.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded mouse brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512432070.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded human colon carcinoma tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512443371.jpg)
![Western Blot: TTK Antibody (3G7) [H00007272-M01] - TTK monoclonal antibody (M01), clone 3G7 Analysis of TTK expression in K-562.](http://images.novusbio.com/fullsize/TTK-Antibody-3G7-Western-Blot-H00007272-M01-img0007.jpg "Western Blot: TTK Antibody (3G7) [H00007272-M01] - TTK monoclonal antibody (M01), clone 3G7 Analysis of TTK expression in K-562.")
![Western Blot: TTK Antibody (3G7) [H00007272-M01] - TTK monoclonal antibody (M01), clone 3G7. Analysis of TTK expression in HeLa.](http://images.novusbio.com/fullsize/TTK-Antibody-3G7-Western-Blot-H00007272-M01-img0006.jpg)
![Western Blot: TTK Antibody (3G7) [H00007272-M01] - Analysis of TTK expression in transfected 293T cell line by TTK monoclonal antibody (M01), clone 3G7.Lane 1: TTK transfected lysate(97.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TTK-Antibody-3G7-Western-Blot-H00007272-M01-img0008.jpg)
![ELISA: TTK Antibody (3G7) [H00007272-M01] - Detection limit for recombinant GST tagged TTK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TTK-Antibody-3G7-Sandwich-ELISA-H00007272-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CHES1 Antibody [NBP2-38866] - Immunofluorescent staining of human cell line RT4 shows localization to nucleus & plasma membrane.](http://images.novusbio.com/fullsize/CHES1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38866-img0004.jpg "Immunocytochemistry/Immunofluorescence: CHES1 Antibody [NBP2-38866] - Immunofluorescent staining of human cell line RT4 shows localization to nucleus & plasma membrane.")
![Immunohistochemistry-Paraffin: CHES1 Antibody [NBP2-38866] - Staining of human lymph node shows distinct cytoplasmic positivity in fractions of cells.](http://images.novusbio.com/fullsize/CHES1-Antibody-Immunohistochemistry-Paraffin-NBP2-38866-img0005.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Cullin 3 Antibody-Western Blot-NBP2-92848-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92848] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Cullin 3 Antibody [NBP2-92848] - Analysis of HeLa cells using Cullin 3 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92848-img0001.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Cullin 3 Antibody [NBP2-92872] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP2-92872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-92872-img0001.jpg)
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/TTK-Antibody-Immunohistochemistry-Paraffin-NBP2-98832-img0002.jpg "Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human TTK in human lung cancer with rabbit polyclonal antibody (1:1000).")
![Immunohistochemistry-Paraffin: TTK Antibody [NBP2-98832] - Staining of human RPS27 in human lung cancer with NBP2-98832 (1:1000).](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-235202511444413.png)
![Immunocytochemistry/Immunofluorescence: TTK Antibody [NBP2-98832] - Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98832 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98832_rabbit-polyclonal-ttk-antibody-23520252135167.png)
![Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: SW620 cell lysate Lane 3: HT-29 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 89 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32232) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32232_rabbit-cullin-3-mab-je37-29-27820241628528.jpg "Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: SW620 cell lysate Lane 3: HT-29 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 89 kDa Observed band size: 75 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32232) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32232) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32232_rabbit-cullin-3-mab-je37-29-298202414115436.jpg)
![Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-278202415212437.jpg "Western Blot: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: PC-12 cell lysate (20 ug/Lane) Lane 4: Mouse spleen tissue lysate (40 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Lane 7: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32233) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cullin 3 Antibody (JE57-80) [NBP3-32233] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Cullin 3 antibody (NBP3-32233) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32233) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32233_rabbit-cullin-3-mab-je57-80-29820241492664.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-88798-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate")
![Immunohistochemistry: Cullin 3 Antibody [NBP2-88798] - Immunohistochemistry with Testis tissue at an antibody concentration of 10ug/ml using anti-CUL3 antibody](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NBP2-88798-img0001.jpg)
![Immunoprecipitation: CHES1 Antibody [NBP2-04051] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FOXN3 antibody NBP2-04051 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FOXN3 was also immunoprecipitated by rabbit anti-FOXN3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/CHES1-Antibody-Immunoprecipitation-NBP2-04051-img0001.jpg "Immunoprecipitation: CHES1 Antibody [NBP2-04051] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FOXN3 antibody NBP2-04051 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FOXN3 was also immunoprecipitated by rabbit anti-FOXN3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")