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![Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB600-487-img0007.jpg "Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NB600-487] - Nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-487-img0005.jpg)
![Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Renilla-Luciferase-Antibody-Western-Blot-NBP2-42914-img0001.jpg "Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.")
![Western Blot: DNCIC1 Antibody [NBP2-16202] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: HL-60 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DNCIC1-Antibody-Western-Blot-NBP2-16202-img0001.jpg "Western Blot: DNCIC1 Antibody [NBP2-16202] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: HL-60 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: DNCIC1 Antibody [NBP2-16202] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNCIC1-Antibody-Immunohistochemistry-Paraffin-NBP2-16202-img0002.jpg)
![Western Blot: PLEKHF2 Antibody [NBP2-19865] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/PLEKHF2-Antibody-Western-Blot-NBP2-19865-img0001.jpg "Western Blot: PLEKHF2 Antibody [NBP2-19865] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: PLEKHF2 Antibody [NBP2-19865] - Breast cancer, using PLEKHF2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PLEKHF2-Antibody-Immunohistochemistry-Paraffin-NBP2-19865-img0002.jpg)
![Western Blot: COL4A3BP Antibody [NBP2-15942] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP2-15942-img0001.jpg "Western Blot: COL4A3BP Antibody [NBP2-15942] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: COL4A3BP Antibody [NBP2-15942] - Paraffin-embedded H441 xenograft. COL4A3BP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunohistochemistry-Paraffin-NBP2-15942-img0002.jpg)
![Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0058.jpg "Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0057.jpg)
![Western Blot: FMO1 Antibody [NBP2-16511] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FMO1-Antibody-Western-Blot-NBP2-16511-img0001.jpg "Western Blot: FMO1 Antibody [NBP2-16511] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![FMO1 antibody [N2C1], Internal detects FMO1 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min.Green: FMO1 protein stained by FMO1 antibody [N2C1], Internal (NBP2-16511) diluted at 1:500.Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/nbp2-16511_rabbit-polyclonal-fmo1-antibody-immunocytochemistry-immunofluorescence-261120251943813.jpg)
![Western Blot: NSMAF Antibody [NBP1-31431] - Analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate. B: NSMAF transfected lysate. 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NSMAF-Antibody-Western-Blot-NBP1-31431-img0004.jpg "Western Blot: NSMAF Antibody [NBP1-31431] - Analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate. B: NSMAF transfected lysate. 7.5% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: NSMAF Antibody [NBP1-31431] - Paraffin-embedded T(gastric CA), using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NSMAF-Antibody-Immunohistochemistry-Paraffin-NBP1-31431-img0003.jpg)
![Western Blot: Keratin 36 Antibody [NBP2-17039] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Keratin-36-Antibody-Western-Blot-NBP2-17039-img0001.jpg "Western Blot: Keratin 36 Antibody [NBP2-17039] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Keratin 36 Antibody [NBP2-17039] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Keratin-36-Antibody-Immunohistochemistry-Paraffin-NBP2-17039-img0002.jpg)
![Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26505_rabbit-apc-mab-5f2-27220248471917.jpg "Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: APC Antibody (5F2) [NBP3-26505] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate. All lanes: APC Antibody at 1:1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 312, 301, 309 kDa Observed band size: 160 kDa](http://images.novusbio.com/fullsize/nbp3-26505_rabbit-apc-mab-5f2-282202411485817.jpg)
![Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP2-22308-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Staufen Antibody [NBP2-22308] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22308 used for IP at 6 ug/mg lysate. Staufen1 was also immunoprecipitated by rabbit anti-Staufen1 antibody. For blotting immunoprecipitated Staufen1, NBP2-22308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP2-22308-img0002.jpg)