| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50407-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27119-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12014-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50222-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81045-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024920238.jpg%3Fv=1666701343 "Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024916246.jpg%3Fv=1666701343)
![Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis of paraffin-embedded testis tissues using ATG14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-104202491703.jpg%3Fv=1666701343)
![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against human ATG14 (AA: 43-303) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024915810.jpg%3Fv=1666701343)
![Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856930.jpg "Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856954.jpg)
![Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-1992023185690.jpg "Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-119202316331615.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL-12-30-Chimeric-Western-Blot-NBP3-12014-img0001.jpg "Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12014 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113044.png)
![Immunocytochemistry /Immunofluorescence: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with NBP3-12014 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12014, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113016.png)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - HaCaTs (keratinocytes) were stained with Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G). Cells were fixed with PFA 4%. Antibody concentration: 1:100. Dilution buffer: 1X PBS, 1% bovine serum albumin (BSA). Image from verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PK110-Immunocytochemistry-Immunofluorescence-NBP2-50222-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - HaCaTs (keratinocytes) were stained with Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G). Cells were fixed with PFA 4%. Antibody concentration: 1:100. Dilution buffer: 1X PBS, 1% bovine serum albumin (BSA). Image from verified customer review.")
![Immunohistochemistry-Frozen: Cytokeratin, pan Antibody (PK110) [NBP2-50222] - Cytokeratin was detected in human skin using Cytokeratin, pan antibody (PK110) [DyLight 488] (NBP2-50222G) (1:100; 1 hour) in PBS. Tissue was fixed in acetone. Image from verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PK110-Immunohistochemistry-Frozen-NBP2-50222-img0001.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-In-vitro-assay-NBP3-14767-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers . A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 uM of fibrils (NBP3-14767) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.")
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0003.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1), A53T Mutant Protein [NBP3-14767] - TEM of A53T alpha synuclein Pre-formed Fibrils (NBP3-14767)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-A53T-Mutant-Protein-Electron-Microscopy-NBP3-14767-img0002.jpg)
![In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14774-img0001.jpg "In vitro assay: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril (NBP3-14774) is the majority of the seed.")
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 500nm](http://images.novusbio.com/fullsize/Recombinant Rat alpha-Synuclein Active- Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14774-img0003.jpg)
![Electron Microscopy: Recombinant Rat alpha-Synuclein Active, Pre-formed Fibrils Protein [NBP3-14774] - TEM of Rat alpha-Synuclein Pre-formed Fibrils (PFFS) (NBP3-14774). 200nm](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14774-img0002.jpg)
![Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Western-Blot-NBP2-81045-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.")
![Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Flow-Cytometry-NBP2-81045-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-81045 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81045_rabbit-monoclonal-beta-2-microglobulin-antibody-bbm-1-chimeric-74202522113026.png)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)