| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-121SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12710-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98561-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98647-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12690-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89210-50ul | |||
![Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.](http://images.novusbio.com/fullsize/SFRP5-Antibody-Western-Blot-NB200-121-img0001.jpg "Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.")
![Western Blot: HYLS1 Antibody [NBP3-12710] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HYLS1-Antibody-Western-Blot-NBP3-12710-img0001.jpg "Western Blot: HYLS1 Antibody [NBP3-12710] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HYLS1 Antibody [NBP3-12710] - Staining of human HYLS1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/HYLS1-Antibody-Immunohistochemistry-Paraffin-NBP3-12710-img0002.jpg)
![Immunoprecipitation: HYLS1 Antibody [NBP3-12710] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-HYLS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HYLS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDa](http://images.novusbio.com/fullsize/HYLS1-Antibody-Immunoprecipitation-NBP3-12710-img0003.jpg)
![Western Blot: UBLCP1 Antibody [NBP2-98561] - Anti-UBLCP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/UBLCP1-Antibody-Western-Blot-NBP2-98561-img0002.jpg "Western Blot: UBLCP1 Antibody [NBP2-98561] - Anti-UBLCP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 43 kDa")
![Immunohistochemistry-Paraffin: UBLCP1 Antibody [NBP2-98561] - Immunochemical staining of human UBLCP1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/UBLCP1-Antibody-Immunohistochemistry-Paraffin-NBP2-98561-img0003.jpg)
![Immunoprecipitation: UBLCP1 Antibody [NBP2-98561] - UBLCP1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-UBLCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBLCP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/UBLCP1-Antibody-Immunoprecipitation-NBP2-98561-img0001.jpg)
![Western Blot: Nephronophthisis Antibody [NBP2-98647] - Anti-Nephronophthisis rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Western-Blot-NBP2-98647-img0004.jpg "Western Blot: Nephronophthisis Antibody [NBP2-98647] - Anti-Nephronophthisis rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa")
![Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-98647] - Immunochemical staining of human Nephronophthisis in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Immunohistochemistry-Paraffin-NBP2-98647-img0003.jpg)
![Immunoprecipitation: Nephronophthisis Antibody [NBP2-98647] - Nephronophthisis was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-Nephronophthisis rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Nephronophthisis rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 80 kDa](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Immunoprecipitation-NBP2-98647-img0001.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PD00-15) [NBP3-32710] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Cytokeratin, pan antibody (NBP3-32710) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32710) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32710_mouse-cytokeratin-pan-mab-pd00-15-298202414262632.jpg "Immunohistochemistry: Cytokeratin, pan Antibody (PD00-15) [NBP3-32710] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Cytokeratin, pan antibody (NBP3-32710) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32710) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PD00-15) [NBP3-32710] - Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin, pan with Mouse anti-Cytokeratin, pan antibody (NBP3-32710) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cytokeratin, pan antibody (NBP3-32710) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32710_mouse-cytokeratin-pan-mab-pd00-15-29202410234239.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (PD00-15) [NBP3-32710] - Flow cytometric analysis of MCF7 cells labeling Cytokeratin, pan. Cells were fixed and permeabilized.Then stained with the primary antibody (NBP3-32710, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32710_mouse-cytokeratin-pan-mab-pd00-15-392024884689.jpg)
![Western Blot: ROGDI Antibody [NBP3-12690] - Lane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: ACHN Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa](http://images.novusbio.com/fullsize/ROGDI-Antibody-Western-Blot-NBP3-12690-img0001.jpg "Western Blot: ROGDI Antibody [NBP3-12690] - Lane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: ACHN Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa")
![Immunohistochemistry-Paraffin: ROGDI Antibody [NBP3-12690] - Staining of human ROGDI in human small intestine with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ROGDI-Antibody-Immunohistochemistry-Paraffin-NBP3-12690-img0002.jpg)
![Immunoprecipitation: ROGDI Antibody [NBP3-12690] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-ROGDI rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ROGDI rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size :32 kDa](http://images.novusbio.com/fullsize/ROGDI-Antibody-Immunoprecipitation-NBP3-12690-img0003.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-288202412461627.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32811) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (NBP3-32811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32811, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241492636.jpg)
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-04) [NBP3-32811] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32811_rabbit-tau-p-thr231-mab-psh01-04-29820241420935.jpg)
![Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target Name: MCOLN1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NBP2-87859-img0004.jpg "Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target Name: MCOLN1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml")
![Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target Name: MCOLN1. Sample Tissue: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NBP2-87859-img0001.jpg)
![Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target Name: MCOLN1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NBP2-87859-img0002.jpg)
![Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target: MCOLN1. Positive control (+): Human Placenta (PL). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NBP2-87859-img0003.jpg)
![Western Blot: Mucolipin 1 Antibody [NBP2-87859] - Host: Rabbit. Target Name: MCOLN1. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NBP2-87859-img0005.jpg)
![Western Blot: STAM-1 Antibody [NBP3-20227] - (1ug/ml) staining of K562 (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NBP3-20227_Western-Blot-STAM-1-Antibody-NBP3-20227-1ug-ml-staining-of-K562-A-and-0-5ug-ml-MCF7-B-cell-lysate-35ug-protein-in-RIPA-buffer-Detected-by-chemiluminescence--5420231130454.jpg "Western Blot: STAM-1 Antibody [NBP3-20227] - (1ug/ml) staining of K562 (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP3-20227_Immunohistochemistry-Paraffin-STAM-1-Antibody-NBP3-20227-6ug-ml-staining-of-paraffin-embedded-Human-Testis-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--5420231130457.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/NBP3-20227_Immunohistochemistry-Paraffin-STAM-1-Antibody-NBP3-20227-Negative-Control-showing-staining-of-paraffin-embedded-Human-Testis-with-no-primary-antibody--5420231130456.jpg)
![Western Blot: ZFX Antibody [NBP2-86421] - Host: Rabbit. Target Name: ZFX. Sample Tissue: Human 293T. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/ZFX-Antibody-Western-Blot-NBP2-86421-img0004.jpg "Western Blot: ZFX Antibody [NBP2-86421] - Host: Rabbit. Target Name: ZFX. Sample Tissue: Human 293T. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry-Paraffin: ZFX Antibody [NBP2-86421] - Rabbit Anti-ZFX Antibody. Paraffin Embedded Tissue: Human alveolar cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/ZFX-Antibody-Immunohistochemistry-Paraffin-NBP2-86421-img0003.jpg)
![Immunohistochemistry-Paraffin: ZFX Antibody [NBP2-86421] - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).. Antibody Concentration: 10 ug/ml.](http://images.novusbio.com/fullsize/ZFX-Antibody-Immunohistochemistry-Paraffin-NBP2-86421-img0001.jpg)
![Immunohistochemistry-Paraffin: ZFX Antibody [NBP2-86421] - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE).. Antibody Concentration: 10 ug/ml.](http://images.novusbio.com/fullsize/ZFX-Antibody-Immunohistochemistry-Paraffin-NBP2-86421-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29820241435295.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunocytochemistry analysis of THP-1 cells labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32162) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202410334075.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Flow cytometric analysis of THP-1 cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32162, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202416453272.jpg)