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![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Western Blot: EPS15 Antibody [NBP3-12544] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/EPS15-Antibody-Western-Blot-NBP3-12544-img0001.jpg "Western Blot: EPS15 Antibody [NBP3-12544] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EPS15-Antibody-Immunohistochemistry-Paraffin-NBP3-12544-img0003.jpg)
![Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human stomach with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EPS15-Antibody-Immunohistochemistry-Paraffin-NBP3-12544-img0002.jpg)
![Immunoprecipitation: EPS15 Antibody [NBP3-12544] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-EPS15 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EPS15 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :150 kDa](http://images.novusbio.com/fullsize/EPS15-Antibody-Immunoprecipitation-NBP3-12544-img0004.jpg)
![Western Blot: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: HCT 116 cell lysate Lane 7: Huh7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-27820241521243.jpg "Western Blot: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: HCT 116 cell lysate Lane 7: Huh7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32246) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-298202414333667.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (6-19-R) [NBP3-32246] - Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 18 with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 18 antibody (NBP3-32246) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32246_mouse-cytokeratin-18-mab-6-19-r-29202410484865.jpg)
![Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-298202414304199.jpg "Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Flow cytometric analysis of HCT 116 cells labeling SAV1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32931, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-392024884625.jpg)
![Western Blot: SAV1 Antibody (PSH04-45) [NBP3-32931] - Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: SW480 cell lysate (20 µg/Lane) Lane 7: BxPC-3 cell lysate (20 µg/Lane) Lane 8: 786-0 cell lysate (negative) (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minute; ECL: 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-3920248453522.jpg)
![Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-2782024163166.jpg "Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-pan Actin antibody (NBP3-32712) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32712) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29820241492695.jpg)
![Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunocytochemistry analysis of HeLa cells labeling pan Actin with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29202410334034.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-288202414191534.jpg "Western Blot: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Western blot analysis of Vimentin on different lysates with Mouse anti-Vimentin antibody (NBP3-33092) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lane 3: L6 cell lysate Lane 4: Daudi cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33092) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Vimentin antibody (NBP3-33092) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33092) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-298202414304186.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (A6-C1-R) [NBP3-33092] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (NBP3-33092) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33092_mouse-vimentin-mab-a6-c1-r-2920241192398.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0005.jpg "Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).")
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0003.jpg)
![Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 knockout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa](http://images.novusbio.com/fullsize/GALK1-Antibody-Western-Blot-NBP2-99783-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 knockout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/GALK1-Antibody-Western-Blot-NBP2-99783-img0007.jpg)
![Immunoprecipitation: GALK1 Antibody [NBP2-99783] - GALK1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-GALK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-GALK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa](http://images.novusbio.com/fullsize/GALK1-Antibody-Immunoprecipitation-NBP2-99783-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GALK1 Antibody [NBP2-99783] - Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99783 antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99783_rabbit-polyclonal-galk1-antibody-235202521525074.png)
![Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-EM-05-Flow-Cell-Surface-NBP1-44763-img0002.jpg "Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.")
![Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human heart showing blood vessel staining (red arrow) (1:200).](http://images.novusbio.com/fullsize/ADAM15-Antibody-007-Immunohistochemistry-Paraffin-NBP2-89546-img0003.jpg "Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human heart showing blood vessel staining (red arrow) (1:200).")
![Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546] - Profile of anti-ADAM15 reactivity on MCF-7 cells.](http://images.novusbio.com/fullsize/ADAM15-Antibody-007-Flow-Cytometry-NBP2-89546-img0001.jpg)
![Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow) (1:2000).](http://images.novusbio.com/fullsize/ADAM15-Antibody-007-Immunohistochemistry-Paraffin-NBP2-89546-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546] - Staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89546 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89546_rabbit-monoclonal-adam15-antibody-007-23520251664726.png)