| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56035-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89624-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44434 | |||
| BSA Free | NBP2-14785-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44085 | |||
| BSA Free | NBP2-52879-25ul | |||
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Western Blot: Phospholemman Antibody - BSA Free [NBP3-47156] - Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (Phospholemman antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-47156_rabbit-phospholemman-pab-bsa-free-284202512283330.jpg "Western Blot: Phospholemman Antibody - BSA Free [NBP3-47156] - Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (Phospholemman antibody) at dilution of 1:200")
![Immunoprecipitation: Phospholemman Antibody - BSA Free [NBP3-47156] - IP Result of anti-Phospholemman/Phospholemman (IP:, 4ug; Detection: 1:500) with mouse heart tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-47156_rabbit-phospholemman-pab-bsa-free-28420251227509.jpg)
![Immunohistochemistry: Phospholemman Antibody - BSA Free [NBP3-47156] - Immunohistochemistry of paraffin-embedded human heart using (Phospholemman antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47156_rabbit-phospholemman-pab-bsa-free-284202512313675.jpg)
![Immunohistochemistry-Paraffin: Urotensin-2 Antibody [NBP3-42365] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 10ug/ml NBP3-42365](http://images.novusbio.com/fullsize/nbp3-42365_rabbit-urotensin-2-pab-6122024205888.jpg "Immunohistochemistry-Paraffin: Urotensin-2 Antibody [NBP3-42365] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 10ug/ml NBP3-42365")
![Western Blot: Urotensin-2 Antibody [NBP3-42365] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-42365_rabbit-urotensin-2-pab-61220242151848.jpg)
![Immunohistochemistry-Paraffin: Urotensin-2 Antibody [NBP3-42365] - DAB staining on IHC-P; Samples: Human Glioma Tissue](http://images.novusbio.com/fullsize/nbp3-42365_rabbit-urotensin-2-pab-612202421121336.jpg)
![Immunoprecipitation: GGCX Antibody - BSA Free [NBP3-46236] - IP Result of anti-GGCX (IP:, 4ug; Detection: 1:2000) with mouse liver tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-46236_rabbit-ggcx-pab-bsa-free-284202511513450.jpg "Immunoprecipitation: GGCX Antibody - BSA Free [NBP3-46236] - IP Result of anti-GGCX (IP:, 4ug; Detection: 1:2000) with mouse liver tissue lysate 4000ug.")
![Western Blot: GGCX Antibody - BSA Free [NBP3-46236] - MCF7 cells were subjected to SDS PAGE followed by western blot with (GGCX antibody) at dilution of 1:2000](http://images.novusbio.com/fullsize/nbp3-46236_rabbit-ggcx-pab-bsa-free-28420251155446.jpg)
![Immunohistochemistry: GGCX Antibody - BSA Free [NBP3-46236] - Immunohistochemistry of paraffin-embedded human liver using (GGCX antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-46236_rabbit-ggcx-pab-bsa-free-284202511513477.jpg)
![Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-298202414333685.jpg "Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32102, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-298202414262618.jpg)
![Western Blot: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Western blot analysis of Calsequestrin 2 on different lysates with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/10,000 dilution.Lane 1: Mouse skeletal muscle tissue lysate (15 µg/Lane)Lane 2: Mouse heart tissue lysate (15 µg/Lane)Lane 3: Mouse brain tissue lysate (negative) (15 µg/Lane)Lane 4: Rat skeletal muscle tissue lysate (15 µg/Lane)Lane 5: Rat heart tissue lysate (15 µg/Lane)Lane 6: Rat brain tissue lysate (negative) (15 µg/Lane)Predicted band size: 46 kDaObserved band size: 55 kDaExposure time: 1 minute 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-3920248453541.jpg)
![Western Blot: DEGS1 Antibody (PSH02-54) [NBP3-32598] - Western blot analysis of DEGS1 on different lysates with Rabbit anti-DEGS1 antibody (NBP3-32598) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32598) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32598_rabbit-degs1-mab-psh02-54-27820241524266.jpg "Western Blot: DEGS1 Antibody (PSH02-54) [NBP3-32598] - Western blot analysis of DEGS1 on different lysates with Rabbit anti-DEGS1 antibody (NBP3-32598) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32598) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DEGS1 Antibody (PSH02-54) [NBP3-32598] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling DEGS1 with Rabbit anti-DEGS1 antibody (NBP3-32598) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32598, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32598_rabbit-degs1-mab-psh02-54-298202414262634.jpg)
![Immunohistochemistry: DEGS1 Antibody (PSH02-54) [NBP3-32598] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-DEGS1 antibody (NBP3-32598) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32598) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32598_rabbit-degs1-mab-psh02-54-298202413585535.jpg)
![Western Blot: EHD1 Antibody [NBP2-56035] - Analysis in human cell line A-431 and human cell line HEK 293.](http://images.novusbio.com/fullsize/EHD1-Antibody-Knockdown-Validated-NBP2-56035-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: EHD1 Antibody [NBP2-56035] - Analysis in human cell line A-431 and human cell line HEK 293.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EHD1 Antibody [NBP2-56035] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56035-img0001.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP1-89624] - Staining of human Stomach shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89624-img0007.jpg "Immunohistochemistry-Paraffin: SCAP Antibody [NBP1-89624] - Staining of human Stomach shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP1-89624] - Staining of human Adrenal gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89624-img0005.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP1-89624] - Staining of human Pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89624-img0006.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP1-89624] - Staining of human Testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89624-img0008.jpg)
![Western Blot: Apolipoprotein L1 Antibody (CL0171) [NBP2-14785] - Lane 1: Marker [kDa], Lane 2: Plasma](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-CL0171-Western-Blot-NBP2-14785-img0003.jpg "Western Blot: Apolipoprotein L1 Antibody (CL0171) [NBP2-14785] - Lane 1: Marker [kDa], Lane 2: Plasma")
![Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody (CL0171) [NBP2-14785] - Staining of human colon shows plasma positivity.](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-CL0171-Immunohistochemistry-Paraffin-NBP2-14785-img0002.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody (CL0171) [NBP2-14785] - Staining of human lung shows plasma positivity.](http://images.novusbio.com/fullsize/Apolipoprotein-L1-Antibody-CL0171-Immunohistochemistry-Paraffin-NBP2-14785-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (CL0210) [NBP2-52879] - Staining of U-87 MG cells using the Anti-NLRP3 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-CL0210-Immunocytochemistry-Immunofluorescence-NBP2-52879-img0002.jpg "Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (CL0210) [NBP2-52879] - Staining of U-87 MG cells using the Anti-NLRP3 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Western Blot: NLRP3/NALP3 Antibody (CL0210) [NBP2-52879] -Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/antibody/nbp2-52879_mouse-monoclonal-nlrp3-nalp3-antibody-cl0210-western-blot-1962024124856..jpg)
