Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0009.jpg "Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.")
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Raji cells with MS4A1/CD20 antibody (MEM-97) NBP1-44634AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0001.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Daudi cells with MS4A1/CD20 (MEM-97) antibody NBP1-44634PE (blue) along with a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0002.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibod](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0006.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Flow Cytometry: MS4A1/CD20 Antibody (MEM-97) [Alexa Fluor 647] [NBP1-44634AF647] - A surface stain was performed on human peripheral blood lymphocytes with MS4A1/CD20 (MEM-97) antibody NBP1-44634AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0007.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Ramos cells with CD20 antibody (MEM-97) NBP1-44634PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0008.jpg)
![Immunoprecipitation: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97)](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Immunoprecipitation-NBP1-44634-img0005.jpg)
![Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Western-Blot-NBP2-67406-img0007.jpg "Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate")
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta-NGF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0006.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0008.jpg "Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0006.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin with Rabbit anti-Actin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0009.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0004.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates with Rabbit anti-Cytokeratin 8 antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Western-Blot-NBP2-67468-img0009.jpg "Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates with Rabbit anti-Cytokeratin 8 antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of SK-Br-3 cells labeling Cytokeratin 8 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 8 (red) at 1/50 dilution and Beta-tubulin (yellow) at 1/500 dilution at +4 overnight. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0012.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Flow cytometric analysis of Cytokeratin 8 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Flow-Cytometry-NBP2-67468-img0008.jpg)
![Western Blot: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Cytokeratin 18 Antibody (SU0338) [NBP2-67468] - Analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Western-Blot-NBP2-67468-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunocytochemistry-Immunofluorescence-NBP2-67468-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Immunohistochemistry-Paraffin-NBP2-67468-img0011.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (SU0338) [NBP2-67468] - Analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-SU0338-Flow-Cytometry-NBP2-67468-img0001.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0008.jpg "Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - ICC staining of Caspase-9 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primar](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0005.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded human tonsil tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0004.jpg)
![Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Western-Blot-NBP2-67463-img0008.jpg "Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0002.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0006.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0013.jpg "Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Immunocytochemistry-Immunofluorescence-H00001958-M03-img0010.jpg)
![Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Immunohistochemistry-Paraffin-H00001958-M03-img0011.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of EGR1 expression in transfected 293T cell line by EGR1 monoclonal antibody (M03), clone 6E8. Lane 1: EGR1 transfected lysatE (59.73 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0012.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-Western-Blot-H00001958-M03-img0014.jpg)
![ELISA: EGR1 Antibody (6E8) [H00001958-M03] - Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EGR1-Antibody-6E8-ELISA-H00001958-M03-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)