| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB5729-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59603-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47881-0.1mg | |||
| IHC-Prediluted | NBP2-48377 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54584-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54524-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54592-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26233-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
![Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Immunocytochemistry-MAB5729-img0002.jpg "Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.")
![Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Flow-Cytometry-MAB5729-img0003.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0009.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained withNSE gamma Monoclonal Antibody (ENO2/1462).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0007.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained withNSE gamma Monoclonal Antibody (ENO2/1462)](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Immunohistochemistry-Paraffin-NBP2-59602-img0008.jpg)
![Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1462-Protein-Array-NBP2-59602-img0012.jpg)
![Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Western-Blot-NBP3-15177-img0005.jpg "Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177] - Immunofluorescence staining of A549 cells with at hnRNP K antibody 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP3-15177-img0001.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0004.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0003.jpg)
![Immunoprecipitation: hnRNP K Antibody (3G7) [NBP3-15177] - Immunoprecipitating hnRNP K in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of hnRNP K antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: hnRNP K antibody (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunoprecipitation-NBP3-15177-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Ovarian Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0006.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Ovarian Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Colon Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/845) [NBP2-44655] - Human Endometrial Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-845-Immunohistochemistry-Paraffin-NBP2-44655-img0005.jpg)
![Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Western-Blot-NBP1-77817-img0002.jpg "Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature")
![Immunocytochemistry/Immunofluorescence: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Used at a 1:500 dilution detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunocytochemistry-Immunofluorescence-NBP1-77817-img0006.jpg)
![Immunohistochemistry: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Analysis of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used at a dilution of 1 to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunohistochemistry-NBP1-77817-img0005.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Western Blot: Ferritin Light Chain Antibody (FTL/1388) [NBP2-53296] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1388).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1388-Western-Blot-NBP2-53296-img0005.jpg "Western Blot: Ferritin Light Chain Antibody (FTL/1388) [NBP2-53296] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1388).")
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1388) [NBP2-53296] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1388).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1388-Immunohistochemistry-Paraffin-NBP2-53296-img0002.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1388) [NBP2-53296] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1388).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1388-Immunohistochemistry-Paraffin-NBP2-53296-img0001.jpg)
![Protein Array: Ferritin Light Chain Antibody (FTL/1388) [NBP2-53296] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin Light Chain Antibody (FTL/1388) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1388-Protein-Array-NBP2-53296-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (CTNG/1664) [NBP2-53172] - Immunofluorescent staining of paraformaldehyde-fixed MCF-7 cells with gamma Catenin Antibody (CTNG/1664) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (Red).](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-CTNG-1664-Immunocytochemistry-Immunofluorescence-NBP2-53172-img0003.jpg "Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (CTNG/1664) [NBP2-53172] - Immunofluorescent staining of paraformaldehyde-fixed MCF-7 cells with gamma Catenin Antibody (CTNG/1664) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (Red).")
![Immunohistochemistry-Paraffin: gamma Catenin Antibody (CTNG/1664) [NBP2-53172] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Catenin, gamma Monoclonal Antibody (CTNG/1664)](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-CTNG-1664-Immunohistochemistry-Paraffin-NBP2-53172-img0002.jpg)
![Immunohistochemistry-Paraffin: gamma Catenin Antibody (CTNG/1664) [NBP2-53172] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Catenin, gamma Monoclonal Antibody (CTNG/1664)](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-CTNG-1664-Immunohistochemistry-Paraffin-NBP2-53172-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0002.jpg)
![Protein Array: WT1 Antibody (rWT1/857) [NBP2-53181] - Analysis of Protein Array containing more than 19,000 full-length human proteins using WT1 Antibody (rWT1/857).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Protein-Array-NBP2-53181-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/nbp2-53181_mouse-monoclonal-wt1-antibody-rwt1-857-1772024938132.jpg)
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-262202415355734.jpg "Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-272202485636.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Positive Western Blot detected in: Ntera-2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate. All lanes: FIH-1/HIF-1AN Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa Observed band size: 36-53 kDa](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202411442647.jpg)
![Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Staining of HepG2 cell with FIH-1/HIF-1AN Antibody (12F11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 525-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-28220241312243.jpg)
![Flow Cytometry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Overlay Peak curve showing Hela cells stained with FIH-1/HIF-1AN Antibody (12F11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202414312744.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)