| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05396-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-61909-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24567-25ul | |||
| Azide and BSA Free | NBP3-43867 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-61908-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26303-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62557-0.025mg | |||
![Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Src [p Tyr529] Antibody-Western Blot-NBP3-05396-img0001.jpg "Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Src [p Tyr529] Antibody - Azide and BSA Free [Src] - Western blot analysis of lysates from A-431 cells, using Src Rabbit pAb at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-05396_rabbit-polyclonal-src-p-tyr529-antibody-52202516293989.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/429) [NBP2-44675] - Immunofluorescent staining of Raji cells. CD19 Antibody (CVID3/429) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-429-Immunocytochemistry-Immunofluorescence-NBP2-44675-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/429) [NBP2-44675] - Immunofluorescent staining of Raji cells. CD19 Antibody (CVID3/429) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Flow Cytometry: CD19 Antibody (CVID3/429) [NBP2-44675] - Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF405S-labeled CD19 antibody (CVID3/429) (violet).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-429-Flow-Cytometry-NBP2-44675-img0004.jpg)
![Flow Cytometry: CD19 Antibody (CVID3/429) [NBP2-44675] - Flow Cytometric Analysis of Raji cells. CD19 Antibody (CVID3/429) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-429-Flow-Cytometry-NBP2-44675-img0002.jpg)
![Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-278202415295551.jpg "Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32199) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-298202413585510.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Flow cytometric analysis of Hela cells labeling HGFR/c-MET. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32199, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-29202416453259.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (PS01-69) [NBP3-32310] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EpCAM/TROP1 antibody (NBP3-32310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32310_rabbit-epcam-trop1-mab-ps01-69-29820241358557.jpg "Immunohistochemistry: EpCAM/TROP1 Antibody (PS01-69) [NBP3-32310] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EpCAM/TROP1 antibody (NBP3-32310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (PS01-69) [NBP3-32310] - Immunocytochemistry analysis of HT-29 cells labeling EpCAM/TROP1 with Rabbit anti-EpCAM/TROP1 antibody (NBP3-32310) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM/TROP1 antibody (NBP3-32310) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32310_rabbit-epcam-trop1-mab-ps01-69-2920241114840.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (PS01-69) [NBP3-32310] - Flow cytometric analysis of HT-29 cells labeling EpCAM/TROP1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32310, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32310_rabbit-epcam-trop1-mab-ps01-69-29202416453273.jpg)
![Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-8-Adhesion-Activation-NBP2-50172-img0001.jpg "Adhesion Activation: HGF R/c-MET Antibody (8) [NBP2-50172] - Epitope position on molecule")
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/155) [NBP2-44674] - Immunofluorescence staining of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Immunocytochemistry-Immunofluorescence-NBP2-44674-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/155) [NBP2-44674] - Immunofluorescence staining of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Flow Cytometry: CD19 Antibody (CVID3/155) [NBP2-44674] - Flow cytometric analysis of lymphocyte-gated PBMCs. CD19 antibody (CVID3/155) labeled with CF568 (orange); unstained cells (gray).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Flow-Cytometry-NBP2-44674-img0004.jpg)
![Flow Cytometry: CD19 Antibody (CVID3/155) [NBP2-44674] - Flow Cytometric Analysis of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Flow-Cytometry-NBP2-44674-img0002.jpg)
![Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate. All lanes: Src Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 61 KDa Observed band size: 60, 61 KDa](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202411472113.jpg "Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate. All lanes: Src Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 61 KDa Observed band size: 60, 61 KDa")
![Immunocytochemistry/Immunofluorescence: Src Antibody (21H5) [NBP3-26303] - Staining of MCF-7 cells with Src Antibody (21H5) at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-28220241312247.jpg)
![Flow Cytometry: Src Antibody (21H5) [NBP3-26303] - Overlay histogram showing SH-SY5Y cells stained with Src Antibody (21H5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202414183653.jpg)
![Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Notch-1-Antibody-E6-Chimeric-Flow-Cytometry-NBP2-62557-img0002.jpg "Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62557_rabbit-monoclonal-notch-1-antibody-e6-chimeric-74202522141235.png)
![Flow Cytometry: CD117/c-kit Antibody (104D2) [NBP3-09161] - (surface staining) of human peripheral blood cells with anti-CD117 (104D2) purified.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-104D2-Flow-Cytometry-NBP3-09161-img0001.jpg "Flow Cytometry: CD117/c-kit Antibody (104D2) [NBP3-09161] - (surface staining) of human peripheral blood cells with anti-CD117 (104D2) purified.")