| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71194 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37822-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95105-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89605-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27107SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47738-0.1mg | |||
| IHC-Prediluted | NBP2-48082 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48504-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-44309SS | |||
![Western Blot: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Western-Blot-NBP2-45448-img0012.jpg "Western Blot: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM.")
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0011.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0002.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0003.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0004.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0005.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0006.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0007.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0008.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0009.jpg)
![Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunohistochemistry-NBP2-45448-img0010.jpg)
![Immunofluorescence: EpCAM/TROP1 Antibody (OTI1H9) [NBP2-45448] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EPCAM.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-OTI1H9-Immunofluorescence-NBP2-45448-img0001.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0011.jpg "Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0013.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0005.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0012.jpg)
![Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-95105-img0006.jpg "Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.")
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638038.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163964.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638034.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251639698.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251636390.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516283218.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638067.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163633.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516345270.jpg)
![Western Blot: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Lane A: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:38 kDa](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Western-Blot-NBP2-89605-img0005.jpg "Western Blot: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Lane A: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:38 kDa")
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Immunocytochemistry-Immunofluorescence-NBP2-89605-img0001.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Immunohistochemistry-Paraffin-NBP2-89605-img0007.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab , then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Flow-Cytometry-NBP2-89605-img0004.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution. Positive staining was localized to membrane of colonic gland epithelium.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Immunohistochemistry-Paraffin-NBP2-89605-img0002.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution. Positive staining was localized to membrane of transitional epithelium.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Immunohistochemistry-Paraffin-NBP2-89605-img0003.jpg)
![Immunoprecipitation: EpCAM/TROP1 Antibody (28) [NBP2-89605] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate Lane D:0.5 mg Caco-2 Whole Cell Lysate 0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-28-Immunoprecipitation-NBP2-89605-img0006.jpg)
![Western Blot: EpCAM/TROP1 Antibody [NBP2-27107] - Analysis of EpCAM using EpCAM antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse NIH 3T3 lysate probed with 3 ug/mL of EpCAM antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-Western-Blot-NBP2-27107-img0006.jpg "Western Blot: EpCAM/TROP1 Antibody [NBP2-27107] - Analysis of EpCAM using EpCAM antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse NIH 3T3 lysate probed with 3 ug/mL of EpCAM antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody [NBP2-27107] - FFPE human colon stained with EpCAM antibody at 1:200, peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-Immunohistochemistry-Paraffin-NBP2-27107-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0002.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0003.jpg)
![Western Blot: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of CD19 on different lysates with Rabbit anti-CD19 antibody at 1/1,000 dilution.Lane 1: Raji cell lysateLane 2: Daudi cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 61 kDaObserved band size: 90 kDaExposure time: 2 minutes;8% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-western-blot-1382024114350..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Daudi cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-138202411462..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Raji cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-1382024114721..jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0012.jpg "Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.")
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0001.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) FITC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0002.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0003.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0004.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0005.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0006.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0007.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0008.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0009.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0010.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0011.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Immunohistochemistry-Paraffin-NBP2-45144-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).")
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Simple Western lane view shows a specific band for c-Myc in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0002.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Electropherogram image of the corresponding Simple Western lane. c-Myc antibody was used at 50 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0005.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Lane view shows a specific band for c-Myc in 0.2 mg/mL of HeLa lysate and antibody at 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 74 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-45144_mouse-monoclonal-c-myc-antibody-9e10-3-1772024959410.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48504-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0014.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48504 (A) shows similar protein distribution across tissues to independent antibody NBP2-48575 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0010.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0013.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [556]. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [556]. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556].](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0004.jpg)
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641] - Human esophageal carcinoma. c-Met antibody [556] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunohistochemistry-Paraffin-NBP2-43641-img0007.jpg)
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg)
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641] - Human breast carcinoma. c-Met antibody [556] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunohistochemistry-Paraffin-NBP2-43641-img0005.jpg)
![Immunoprecipitation: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of MET protein from HeLa whole cell extracts using 5 ug of MET antibody [556]. Western blot analysis was performed using MET antibody [556]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Immunoprecipitation-NBP2-43641-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44309-img0003.jpg "Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - IHC analysis of HGF R/c-Met (1G7NB) in human liver cancer.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44309-img0004.jpg)
![Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [Azide Free] [NBP2-44309] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Flow-Intracellular-NBP2-44309-img0002.jpg)