| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38321-25ul | |||
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder. " alt="CD21 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Rabbit Anti-Human CD21, Monoclonal Antibody (Catalog # NBP3-20354) at 1:2000 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21126 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84596-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88316-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16894-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03498-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54355-100ug | |||
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining in human placenta and tonsil tissues . Corresponding CD34 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining in human placenta and tonsil tissues . Corresponding CD34 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human kidney shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human placenta shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human testis shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human tonsil shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0007.jpg)
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Analysis in human lung and skeletal muscle tissues. Corresponding CD93 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Analysis in human lung and skeletal muscle tissues. Corresponding CD93 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Staining of human lung shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0012.jpg)
![Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Staining of human skeletal muscle shows moderate membranous positivity in endothelial cells, myocytes are negative.](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Staining of human colon, kidney, lung and skeletal muscle using Anti-CD93 antibody NBP1-88316 (A) shows similar protein distribution across tissues to independent antibody NBP1-88317 (B).](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0009.jpg)
![Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Staining of human kidney shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0011.jpg)
![Immunohistochemistry-Paraffin: C1qR1/CD93 Antibody [NBP1-88316] - Staining of human colon shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/C1q-R1-CD93-Antibody-Immunohistochemistry-Paraffin-NBP1-88316-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunocytochemistry-Immunofluorescence-NBP3-07754-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunohistochemistry-Paraffin-NBP3-07754-img0003.jpg)
![Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Flow-Cytometry-NBP3-07754-img0001.jpg)
![Western Blot: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Western blot analysis of extracts of MDA-MB435 cells, using NFkB2/NFkB p100 antibody (NBP3-16894) at dilution. MDA-MB435 cells were treated by TNF-alpha. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/NFkB2-NFkB-p100-[p-Ser866]-Antibody-Western-Blot-NBP3-16894-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Western blot analysis of extracts of MDA-MB435 cells, using NFkB2/NFkB p100 antibody (NBP3-16894) at dilution. MDA-MB435 cells were treated by TNF-alpha. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Immunofluorescence analysis of methanol-fixed HeLa cells using NFkB2/NFkB p100 antibody (NBP3-16894).](http://images.novusbio.com/fullsize/NFkB2-NFkB-p100-[p-Ser866]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16894-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 [p Ser866] Antibody [NBP3-16894] - Immunohistochemistry of paraffin-embedded human breast carcinoma using NFkB2/NFkB p100 antibody (NBP3-16894).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NFkB2-NFkB-p100-[p-Ser866]-Antibody-Immunohistochemistry-Paraffin-NBP3-16894-img0001.jpg)
![Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-immunoprecipitation-711202482349..png "Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.")
![Western Blot: IP3R3 Antibody [NBP3-03498] - Analysis of lysates from HeLa cells, using ITPR3 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-western-blot-711202482521..png)
![Immunocytochemistry/ Immunofluorescence: IP3R3 Antibody - Azide and BSA Free [IP3R3] - Immunofluorescence analysis of NIH-3T3 cells using IP3R3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-5220251640284.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with NBP3-43538 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43538, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43538_mouse-mhc-class-ii-mab-ox-6-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2220260351121.png "Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with NBP3-43538 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43538, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.")
![Western Blot: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43538 at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for MHC Class II is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43538 successfully detected MHC Class II in rat spleen, thymus and lymph node lysates.](http://images.novusbio.com/fullsize/nbp3-43538_mouse-mhc-class-ii-mab-ox-6-azide-and-bsa-free-western-blot-2220260333112.png)
![Flow Cytometry: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43538 at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43538_mouse-mhc-class-ii-mab-ox-6-azide-and-bsa-free-flow-cytometry-2220260325820.png)
![Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-SPM494-Flow-Cytometry-NBP2-44744-img0002.jpg "Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)](http://images.novusbio.com/fullsize/CD20-Antibody-L26-+-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44747-img0001.jpg "Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Antibody (HPCA1/1806R).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Immunohistochemistry-Paraffin-NBP2-53353-img0002.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Antibody (HPCA1/1806R).")
![Flow Cytometry: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Flow Cytometric Analysis of human Jurkat cells using CD34 Antibody (HPCA1/1806R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Flow-Cytometry-NBP2-53353-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Immunohistochemistry-Paraffin-NBP2-53353-img0001.jpg)
