| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38434-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62026B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25972 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38321-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62615-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26871-25ul | |||
| Azide and BSA Free | NBP3-43916 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15134-25ul | |||
| Azide and BSA Free | NBP3-44363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15135-25ul | |||
| Azide and BSA Free | NBP3-44364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43426-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52682-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody [NBP2-38434] - Staining in human tonsil and cerebral cortex tissues using anti-CD27 antibody. Corresponding CD27 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-Immunohistochemistry-Paraffin-NBP2-38434-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody [NBP2-38434] - Staining in human tonsil and cerebral cortex tissues using anti-CD27 antibody. Corresponding CD27 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD27/TNFRSF7 Antibody [NBP2-38434] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line Daudi](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-Western-Blot-NBP2-38434-img0002.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody [NBP2-38434] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-Immunohistochemistry-Paraffin-NBP2-38434-img0004.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody [NBP2-38434] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-Immunohistochemistry-Paraffin-NBP2-38434-img0005.jpg)
![Immunohistochemistry: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62026.](http://images.novusbio.com/fullsize/IgD-Antibody-RM123-Unconjugated-Immunohistochemistry-NBP2-62026-img0001.jpg "Immunohistochemistry: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62026.")
![ELISA: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - ELISA of human immunoglobulins shows NBP2-62026 reacts to IgD from human plasma and IgD kappa from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with different immunoglobulins.NBP2-62026 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/IgD-Antibody-RM123-Unconjugated-ELISA-NBP2-62026-img0005.jpg)
![ELISA: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - Sandwich ELISA using NBP2-62026 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody (NBP2-77396) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/IgD-Antibody-RM123-Unconjugated-ELISA-NBP2-62026-img0007.jpg)
![ELISA: IgD Antibody (RM123) [Unconjugated] [NBP2-62026] - A titer ELISA using NBP2-62026. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of NBP2-62026 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/IgD-Antibody-RM123-Unconjugated-ELISA-NBP2-62026-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining in human placenta and tonsil tissues . Corresponding CD34 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining in human placenta and tonsil tissues . Corresponding CD34 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human kidney shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human placenta shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human testis shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38321] - Staining of human tonsil shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38321-img0007.jpg)
![Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Analysis in human tonsil and cerebral cortex tissues. Corresponding FCRL3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FCRL3-FcRH3-Antibody-Immunohistochemistry-Paraffin-NBP2-62615-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Analysis in human tonsil and cerebral cortex tissues. Corresponding FCRL3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Staining of human small intestine shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/FCRL3-FcRH3-Antibody-Immunohistochemistry-Paraffin-NBP2-62615-img0005.jpg)
![Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Staining of human tonsil shows positivity in lymphoid cells.](http://images.novusbio.com/fullsize/FCRL3-FcRH3-Antibody-Immunohistochemistry-Paraffin-NBP2-62615-img0007.jpg)
![Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Staining of human cerebral cortex shows no positivity as expected.](http://images.novusbio.com/fullsize/FCRL3-FcRH3-Antibody-Immunohistochemistry-Paraffin-NBP2-62615-img0008.jpg)
![Immunohistochemistry-Paraffin: FCRL3/FcRH3 Antibody [NBP2-62615] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-62615_rabbit-polyclonal-fcrl3-fcrh3-antibody-immunohistochemistry-paraffin-232202412923..jpg)
![Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-CL12435-Immunohistochemistry-Paraffin-NBP3-15134-img0004.jpg "Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Staining of human chronic lymphocytic leukemia shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-CL12435-Immunohistochemistry-Paraffin-NBP3-15134-img0001.jpg)
![Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-CL12435-Immunohistochemistry-Paraffin-NBP3-15134-img0002.jpg)
![Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-CL12435-Immunohistochemistry-Paraffin-NBP3-15134-img0003.jpg)
![Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (CL12435) [NBP3-15134] - Analysis in human lymph node and skeletal muscle tissues using NBP3-15134 antibody. Corresponding FCER2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-CL12435-Immunohistochemistry-Paraffin-NBP3-15134-img0005.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0004.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human kidney shows strong apical membranous positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0002.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human skin shows very weak positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Analysis in human small intestine and pancreas tissues using NBP3-15135 antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0005.jpg)
![Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody [GT4811] (NBP3-13508) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-GT4811-Western-Blot-NBP3-13508-img0004.jpg "Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody [GT4811] (NBP3-13508) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-GT4811-Western-Blot-NBP3-13508-img0001.jpg)
![Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-GT4811-Western-Blot-NBP3-13508-img0002.jpg)
![Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-GT4811-Western-Blot-NBP3-13508-img0003.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the PE/Cy7 conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (open histogram) or Anti-Human CD27 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0011.jpg "Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the PE/Cy7 conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (open histogram) or Anti-Human CD27 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.")
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the Allophycocyanin conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (open histogram) or Anti-Human CD27 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0006.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the Biotin conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Anti-Human CD27 PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0007.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the FITC conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Anti-Human CD19 APC and Mouse IgG1 K Isotype Control FITC (left) or Anti-Human CD27 FITC (right). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0008.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the PE conjugate of NBP1-43426. Analysis of human peripheral blood leukocytes using CD27 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0009.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the PE/Cy5 conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy5 (open histogram) or 0.25 ug of Anti-Human CD27 PE-Cy5 (filled histogram).=](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-O323-Flow-Cytometry-NBP1-43426-img0010.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Immunohistochemistry-NBP2-52682-img0001.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Flow-Cytometry-NBP2-52682-img0002.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)