| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1543-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98994-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52655-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20094-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61611-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27153-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47833-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48291 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11576 | |||
![Western Blot: BNIP3 Antibody (ANa40) [NB100-1543] - Monoclonal Anti-BNIP3 antibody produced in mouse](http://images.novusbio.com/fullsize/BNIP3-Antibody-ANa40-Western-Blot-NB100-1543-img0001.jpg "Western Blot: BNIP3 Antibody (ANa40) [NB100-1543] - Monoclonal Anti-BNIP3 antibody produced in mouse")
![Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-288202412442987.jpg "Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg)
![Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-278202416285257.jpg "Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (JE30-61) [NBP3-32056] - Immunocytochemistry analysis of HCT 116 cells labeling ATG12 with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-2920241192327.jpg)
![Flow Cytometry: ATG12 Antibody (JE30-61) [NBP3-32056] - Flow cytometric analysis of HCT 116 cells labeling ATG12. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32056, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-29202416453286.jpg)
![Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-98994-img0003.jpg "Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-98994] - Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98994-img0002.jpg)
![Immunoprecipitation: TSG101 Antibody [NBP2-98994] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TSG101 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunoprecipitation-NBP2-98994-img0004.jpg)
![Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856958.jpg "Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).")
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856971.jpg)
![Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924252.png "Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-16D-6B9 (NBP3-20094) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20094, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924253.png)
![Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).](http://images.novusbio.com/fullsize/TNF-alpha-Variant-Antibody-Z74P2C5-A12-Immunohistochemistry-NBP2-61611-img0001.jpg "Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).")
![Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-61611_mouse-tnf-alpha-mab-z74p2c5-a12-bsa-free-20920231412571.jpg)
![ELISA: WDFY3 Antibody (4G9H4) [NBP3-27153] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27153_mouse-wdfy3-mab-4g9h4-bsa-free-1042024915818.jpg%3Fv=1666704387 "ELISA: WDFY3 Antibody (4G9H4) [NBP3-27153] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![Western Blot: WDFY3 Antibody (4G9H4) [NBP3-27153] - Analysis using WDFY3 mAb against human WDFY3 (AA: 3277-3526) recombinant protein. (Expected MW is 54.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27153_mouse-wdfy3-mab-4g9h4-bsa-free-104202491627.jpg%3Fv=1666704387)
![Western Blot: WDFY3 Antibody (4G9H4) [NBP3-27153] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27153_mouse-wdfy3-mab-4g9h4-bsa-free-104202491628.jpg%3Fv=1666704387)
![Flow Cytometry: WDFY3 Antibody (4G9H4) [NBP3-27153] - Analysis of Hela cells using WDFY3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27153_mouse-wdfy3-mab-4g9h4-bsa-free-1042024920237.jpg%3Fv=1666704387)
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM203) - IHC-Prediluted [NBP2-48236] - Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM203).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM203-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48236-img0001.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (SPM203) - IHC-Prediluted [NBP2-48236] - Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM203).")
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) - Azide and BSA Free [NBP2-47668] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM145-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47668-img0001.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (SPM145) - Azide and BSA Free [NBP2-47668] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).")
![Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) - IHC-Prediluted [NBP2-48293] - Human cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).](http://images.novusbio.com/fullsize/NF-L-Antibody-SPM204-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48293-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) - IHC-Prediluted [NBP2-48293] - Human cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).")