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![Immunohistochemistry: ER alpha/NR3A1 Antibody (PD00-04) [NBP3-32321] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-ER alpha/NR3A1 antibody (NBP3-32321) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32321_rabbit-er-alpha-nr3a1-mab-pd00-04-298202413585568.jpg "Immunohistochemistry: ER alpha/NR3A1 Antibody (PD00-04) [NBP3-32321] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-ER alpha/NR3A1 antibody (NBP3-32321) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: FGF18 Antibody (PSH01-25) [NBP3-32337] - Western blot analysis of FGF18 on different lysates with Rabbit anti-FGF18 antibody (NBP3-32337) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T overexpress mouse FGF18 whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32337) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32337_rabbit-fgf18-mab-psh01-25-278202415295559.jpg "Western Blot: FGF18 Antibody (PSH01-25) [NBP3-32337] - Western blot analysis of FGF18 on different lysates with Rabbit anti-FGF18 antibody (NBP3-32337) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T overexpress mouse FGF18 whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32337) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IL-18/IL-1F4 Antibody (PSH02-09) [NBP3-32452] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32452) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: Raji cell lysate (low expression) Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32452) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32452_rabbit-il-18-il-1f4-mab-psh02-09-278202416331248.jpg "Western Blot: IL-18/IL-1F4 Antibody (PSH02-09) [NBP3-32452] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32452) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: Raji cell lysate (low expression) Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32452) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aminopeptidase N/CD13 Antibody (PD00-39) [NBP3-32123] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Aminopeptidase N/CD13 antibody (NBP3-32123) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32123_rabbit-aminopeptidase-n-cd13-mab-pd00-39-298202414161827.jpg "Immunohistochemistry: Aminopeptidase N/CD13 Antibody (PD00-39) [NBP3-32123] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Aminopeptidase N/CD13 antibody (NBP3-32123) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: FGF18 Antibody [NBP2-84022] - WB Suggested Anti-FGF18 Antibody. Titration: 1.0 ug/ml. Positive Control: ACHN Whole Cell](http://images.novusbio.com/fullsize/FGF18-Antibody-Western-Blot-NBP2-84022-img0001.jpg "Western Blot: FGF18 Antibody [NBP2-84022] - WB Suggested Anti-FGF18 Antibody. Titration: 1.0 ug/ml. Positive Control: ACHN Whole Cell")
![Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody [NBP2-97892] - Immunochemical staining of human TEM7/PLXDC1 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TEM7-PLXDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-97892-img0001.jpg "Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody [NBP2-97892] - Immunochemical staining of human TEM7/PLXDC1 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: Cystatin F Antibody [NBP2-99007] - Anti-Cystatin F rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Western-Blot-NBP2-99007-img0002.jpg "Western Blot: Cystatin F Antibody [NBP2-99007] - Anti-Cystatin F rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa")
![Immunohistochemistry-Paraffin: BAI3 Antibody [NBP3-06666] - Human brain, cerebellum tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/BAI3-Antibody-Immunohistochemistry-Paraffin-NBP3-06666-img0001.jpg "Immunohistochemistry-Paraffin: BAI3 Antibody [NBP3-06666] - Human brain, cerebellum tissue after heat-induced antigen retrieval.")
![Western Blot: TBX4 Antibody [NBP2-85890] - Host: Rabbit. Target Name: Tbx4. Sample Type: Rat Spleen lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/TBX4-Antibody-Western-Blot-NBP2-85890-img0001.jpg "Western Blot: TBX4 Antibody [NBP2-85890] - Host: Rabbit. Target Name: Tbx4. Sample Type: Rat Spleen lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: FHL2 Antibody [NBP3-30885] - Western blot using Novus Biologicals' NBP3-30885, rabbit polyclonal at 0.4 ug/ml on HeLa cell extract (10 ug/lane). Blots were developed with goat anti-rabbit Ig (1:75k) and Pierce’s Supersignal West Femto system. Lane: 1- NBP3-30885 at 0.4 ug/ml. 2- NBP3-30885 plus 16 ug blocking peptide. 3 - Goat anti Rabbit HRP control.](http://images.novusbio.com/fullsize/nbp3-30885_rabbit-fhl2-pab-81120242059427.jpg "Western Blot: FHL2 Antibody [NBP3-30885] - Western blot using Novus Biologicals' NBP3-30885, rabbit polyclonal at 0.4 ug/ml on HeLa cell extract (10 ug/lane). Blots were developed with goat anti-rabbit Ig (1:75k) and Pierce’s Supersignal West Femto system. Lane: 1- NBP3-30885 at 0.4 ug/ml. 2- NBP3-30885 plus 16 ug blocking peptide. 3 - Goat anti Rabbit HRP control.")
![Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT Antibody-Immunohistochemistry-Paraffin-NBP3-14395-img0001.jpg "Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Amylin Antibody [NBP3-14540] - Human Pancreatic Islets of Langerhans: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Amylin Antibody-Immunohistochemistry-Paraffin-NBP3-14540-img0001.jpg "Immunohistochemistry-Paraffin: Amylin Antibody [NBP3-14540] - Human Pancreatic Islets of Langerhans: Formalin-Fixed, Paraffin-Embedded (FFPE)")