HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK001 human epidermal keratinocyte cell line, WS-1 human fetal skin fibroblast cell line, U-118-MG human glioblastoma/astrocytoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glis1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7618) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />