| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB17761-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1205-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1387-SP | |||
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Immunohistochemistry-Paraffin-NBP3-13678-img0002.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-9102024204127.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020242013406.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) PC-12 whole cell extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020241959351.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-western-blot-261120251837507.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)
![Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBP2-05603] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.](http://images.novusbio.com/fullsize/JNK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05603-img0001.jpg "Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBP2-05603] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.")
![Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBL1-12884] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.](http://images.novusbio.com/fullsize/JNK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12884-img0002.jpg "Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBL1-12884] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.")
![Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBP2-05602] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.](http://images.novusbio.com/fullsize/JNK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05602-img0001.jpg "Western Blot: JNK1 Overexpression Lysate (Adult Normal) [NBP2-05602] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JNK1.")
![SDS-Page: Recombinant Human JNK1 Protein [NBP2-52123]](http://images.novusbio.com/fullsize/Recombinant-Human-JNK1-Protein-SDS-Page-NBP2-52123-img0002.jpg "SDS-Page: Recombinant Human JNK1 Protein [NBP2-52123]")
