10133-B1)." class="big_lightbox"> 10133-B1)." alt="Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.03-0.15 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80 Fc Chimera (10133-B1)." class="big_thumb" />

HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox"> AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

140-B1)." class="big_lightbox"> 140-B1)." alt="Recombinant Mouse CTLA-4 Fc Chimera inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.03-0.15 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1)." class="big_thumb" />

140-B1) in the presence of PHA." class="big_lightbox"> 140-B1) in the presence of PHA." alt="Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA." class="big_thumb" />

434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." class="big_lightbox"> 434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." alt="Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." class="big_thumb" />

434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />