| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4909-SP | |||
| Carrier Free | FAB4909V-100UG | |||
| Carrier Free | FAB4909U-100UG | |||
| Carrier Free | FAB4909T-100UG | |||
| Carrier Free | FAB4909S-100UG | |||
| Carrier Free | FAB4909R-100UG | |||
| Carrier Free | FAB4909N-100UG | |||
| Carrier Free | FAB4909G-100UG | |||
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD21 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human CD21, Monoclonal Antibody (Catalog # NBP3-26866) at 1:800 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26866-25ul | |||
| Azide and BSA Free | NBP3-44288 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4909-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38895-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38538-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26867-25ul | |||
| Azide and BSA Free | NBP3-44289 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21126 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38684-25ul | |||
![Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Western-Blot-NBP2-67605-img0006.jpg "Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0001.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0003.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0004.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0007.jpg)
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527] - Human tonsil tissue section stained with CD21 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD21-Antibody-1F8-Immunohistochemistry-Paraffin-NBP1-22527-img0003.jpg "Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527] - Human tonsil tissue section stained with CD21 antibody. IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527] - Formalin fixed paraffin embedded human tonsil stained with CD21 antibody (NBP1-22527).](http://images.novusbio.com/fullsize/CD21-Antibody-1F8-Immunohistochemistry-Paraffin-NBP1-22527-img0002.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium, kidney, lymph node and tonsil using Anti-CD21 antibody NBP2-38895 (A) shows similar protein distribution across tissues to independent antibody NBP2-38684 (B).](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0010.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] -Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38895-img0011.jpg)
![Western Blot: CD21 Antibody [NBP2-38895] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CD21-Antibody-Western-Blot-NBP2-38895-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0006.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0008.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human kidney shows very weak membranous positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0009.jpg)
![Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-9122024159579.jpg "Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded human spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171172.jpg)
![Western Blot: CD21 Antibody [NBP3-38538] - Western blot analysis of various lysates using CD21 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202414163018.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded mouse spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415121332.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171157.jpg)
![Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-278202415295561.jpg "Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD21 Antibody (PD00-23) [NBP3-32135] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD21 antibody (NBP3-32135) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32135) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-298202413585521.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody (PD00-23) [NBP3-32135] - Immunocytochemistry analysis of Raji cells labeling CD21 with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202410484856.jpg)
![Flow Cytometry: CD21 Antibody (PD00-23) [NBP3-32135] - Flow cytometric analysis of Raji cells labeling CD21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32135, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202416453281.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunocytochemistry-Immunofluorescence-NBP3-07754-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Immunohistochemistry-Paraffin-NBP3-07754-img0003.jpg)
![Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD21-Antibody-CR2-3124R-Flow-Cytometry-NBP3-07754-img0001.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human endometrium, kidney, lymph node and tonsil using Anti-CD21 antibody NBP2-38684 (A) shows similar protein distribution across tissues to independent antibody NBP2-38895 (B).](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0011.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0012.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human kidney shows very weak membranous positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0013.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38684-img0014.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38684] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38684-img0015.jpg)
