![Immunocytochemistry/Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Immunofluorescent analysis of arboviruses infected cells using Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587). Sample: Mock and zika virus-infected cells. Green: Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587) diluted at 1:100.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Immunocytochemistry-Immunofluorescence-NBP3-13587-img0008.jpg "Immunocytochemistry/Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Immunofluorescent analysis of arboviruses infected cells using Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587). Sample: Mock and zika virus-infected cells. Green: Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587) diluted at 1:100.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | GT363 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika Virus (H/PF/2013) Envelope (Zika virus (strain H/PF/2013)). The exact sequence is proprietary. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Immunofluorescent analysis of non-infected and infected vero cells using Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587). Green: Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587) diluted at 1:500.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Immunocytochemistry-Immunofluorescence-NBP3-13587-img0002.jpg "Immunocytochemistry/Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Immunofluorescent analysis of non-infected and infected vero cells using Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587). Green: Zika Virus (H/PF/2013) Envelope antibody [GT363] (NBP3-13587) diluted at 1:500.")
![Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13205 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Sandwich-ELISA-NBP3-13587-img0003.jpg "Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13205 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.")
![Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13206 as capture antibody at concentration of 5 ug/mL and NBP3-13587 as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Sandwich-ELISA-NBP3-13587-img0004.jpg "Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13206 as capture antibody at concentration of 5 ug/mL and NBP3-13587 as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.")
![Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13206 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Sandwich-ELISA-NBP3-13587-img0005.jpg "Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13206 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.")
![Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of Zika virus-infected lysate using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13206 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-Virus-H-PF-2013-Envelope-Antibody-GT363-Sandwich-ELISA-NBP3-13587-img0006.jpg "Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody (GT363) [NBP3-13587] - Sandwich ELISA detection of Zika virus-infected lysate using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13206 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.")
![Zika Virus-PRVABC59 infected Vero cells were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (HRP) (NBP3-13587-01) diluted at 1:2000.](http://images.novusbio.com/fullsize/nbp3-13587_mouse-zika-virus-h-pf-2013-envelope-mab-gt363-western-blot-2611202519912.jpg "Zika Virus-PRVABC59 infected Vero cells were separated by 4-20% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (HRP) (NBP3-13587-01) diluted at 1:2000.")
![Zika virus Viral Lysate (0.5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (HRP) (NBP3-13587-01) diluted at 1:2000.](http://images.novusbio.com/fullsize/nbp3-13587_mouse-zika-virus-h-pf-2013-envelope-mab-gt363-western-blot-261120251828356.jpg "Zika virus Viral Lysate (0.5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (HRP) (NBP3-13587-01) diluted at 1:2000.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (NBP3-13587) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13587_mouse-zika-virus-h-pf-2013-envelope-mab-gt363-western-blot-26112025197455.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (NBP3-13587) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
