XAGE1D Antibody

Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997] - (3.8ug/ml) IHC staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

XAGE1D Antibody Summary

• Description: Novus Biologicals Goat XAGE1D Antibody (NB300-997) is a polyclonal antibody validated for use in IHC and ELISA. Anti-XAGE1D Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CGFGFRRQGEDNT corresponding to C-Terminus according to NP_597673.1.
• Specificity: This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XAGE1D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as small as the predicted MW of approx. 8.0 kDa band according to NP_597673. IHC-P: Human testis shows polar cytoplasm staining in primary spermatocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XAGE1D Antibody

• cancer/testis associated protein
• CT12.1C
• CTP9
• G antigen family D member 2
• member 1c
• X antigen family, member 1C
• XAGE1

Background

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 3 transcript variants, and one of which includes 2 transcripts generated from alternate transcription initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XAGE1D Antibody (NB300-997)(1)

Publication using NB300-997

• Liu XF, Helman LJ, Yeung C et al. XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma. Cancer Res 2000-09-01 [PMID: 10987281]

Bioinformatics

• Gene Symbol: XAGE1D
• Entrez:
  • 9503(Human)
• Uniprot:
  • Q9HD64(Human)