XAGE1D Antibody

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Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997] - (3.8ug/ml) IHC staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Concentration
0.5 mg/ml

Order Details

XAGE1D Antibody Summary

Immunogen
Peptide with sequence CGFGFRRQGEDNT corresponding to C-Terminus according to NP_597673.1.
Specificity
This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
Clonality
Polyclonal
Host
Goat
Gene
XAGE1D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as small as the predicted MW of approx. 8.0 kDa band according to NP_597673. IHC-P: Human testis shows polar cytoplasm staining in primary spermatocytes.
Publications
Read Publication using NB300-997.

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for XAGE1D Antibody

  • cancer/testis associated protein
  • CT12.1C
  • CTP9
  • G antigen family D member 2
  • member 1c
  • X antigen family, member 1C
  • XAGE1

Background

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 3 transcript variants, and one of which includes 2 transcripts generated from alternate transcription initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XAGE1D Antibody (NB300-997)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol XAGE1D
Entrez
Uniprot

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