wnvNS3 Protease Antibody (345713) [Unconjugated]

Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB2907) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 345713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

wnvNS3 Protease Antibody (345713) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant West Nile Virus NS3 Protease, expressed as a fusion protein with co-factor NS2L
  Gly1506-Leu1689
  Accession # ABD85079
• Specificity: Detects viral wnvNS3 Protease in direct ELISAs and Western blots. Recognizes an epitope in the NS3 domain.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLY
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody (345713) [Unconjugated]

• wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the co‑factor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for wnvNS3 Protease Antibody (MAB2907)(2)

Publications using MAB2907

• Genoyer, E;Wilson, J;Ames, JM;Stokes, C;Moreno, D;Etzyon, N;Oberst, A;Gale, M; Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus bioRxiv : the preprint server for biology 2024-06-07 [PMID: 38895355] (Immunocytochemistry, Human)
• Xu Z, Anderson R, Hobman TC The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus. J. Virol., 2011-03-16;85(11):5571-80. 2011-03-16 [PMID: 21411523] (ICC, Human)

Bioinformatics

• Gene Symbol: POLY
• Uniprot:
  • ABD85079(Viral)