| Reactivity | VSpecies Glossary |
| Applications | WB |
| Clone | 345713 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 350 |
| Immunogen | E. coli-derived recombinant West Nile Virus NS3 Protease, expressed as a fusion protein with co-factor NS2L Gly1506-Leu1689 Accession # ABD85079 |
| Specificity | Detects viral wnvNS3 Protease in direct ELISAs and Western blots. Recognizes an epitope in the NS3 domain. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity Statement | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied |
| Buffer | Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide |
Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the co‑factor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.
Secondary Antibodies |
Isotype Controls |
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